+Open data
-Basic information
Entry | Database: PDB / ID: 3swy | ||||||
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Title | CNGA3 626-672 containing CLZ domain | ||||||
Components | Cyclic nucleotide-gated cation channel alpha-3 | ||||||
Keywords | TRANSPORT PROTEIN / Coiled-coil / Assembly domain | ||||||
Function / homology | Function and homology information inorganic cation import across plasma membrane / intracellular cyclic nucleotide activated cation channel complex / intracellularly cGMP-activated cation channel activity / intracellularly cAMP-activated cation channel activity / axon initial segment / transmembrane transporter complex / myosin binding / monoatomic cation transmembrane transport / glial cell projection / ligand-gated monoatomic ion channel activity ...inorganic cation import across plasma membrane / intracellular cyclic nucleotide activated cation channel complex / intracellularly cGMP-activated cation channel activity / intracellularly cAMP-activated cation channel activity / axon initial segment / transmembrane transporter complex / myosin binding / monoatomic cation transmembrane transport / glial cell projection / ligand-gated monoatomic ion channel activity / response to magnesium ion / monoatomic cation transport / cGMP binding / response to cAMP / visual perception / perikaryon / cadherin binding / dendrite / protein-containing complex binding / signal transduction / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.9 Å | ||||||
Authors | Shuart, N.G. / Haitin, Y. / Camp, S.S. / Black, K.D. / Zagotta, W.N. | ||||||
Citation | Journal: Nat Commun / Year: 2011 Title: Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels. Authors: Shuart, N.G. / Haitin, Y. / Camp, S.S. / Black, K.D. / Zagotta, W.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3swy.cif.gz | 66.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3swy.ent.gz | 51 KB | Display | PDB format |
PDBx/mmJSON format | 3swy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sw/3swy ftp://data.pdbj.org/pub/pdb/validation_reports/sw/3swy | HTTPS FTP |
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-Related structure data
Related structure data | 3swfC 1zvbS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 5246.968 Da / Num. of mol.: 3 / Fragment: CLZ domain (UNP residues 626-669) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CNCG3, CNGA3 / Plasmid: pETM11 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q16281 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.59 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: 20% w/v PEG3350, 200 mM potassium acetate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 77 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 28, 2010 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Double Crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.9→46.324 Å / Num. all: 10016 / Num. obs: 10016 / % possible obs: 98.6 % / Redundancy: 3.1 % / Biso Wilson estimate: 27.698 Å2 / Rsym value: 0.05 / Net I/σ(I): 9.6 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1ZVB Resolution: 1.9→29.217 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.7997 / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 26.73 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.61 Å / VDW probe radii: 0.9 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 53.858 Å2 / ksol: 0.401 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 117.37 Å2 / Biso mean: 31.3853 Å2 / Biso min: 9.89 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→29.217 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 3
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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