温度: 297 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 詳細: 20 mg/mL protein (in 10 mM BTP pH 7.5) mixed 1:1 with reservoir liquor containing 100 mM NaOAc (pH 4.6) and 0.6-3.0 M NaCl. Immediately prior to data collection, crystals were harvested and ...詳細: 20 mg/mL protein (in 10 mM BTP pH 7.5) mixed 1:1 with reservoir liquor containing 100 mM NaOAc (pH 4.6) and 0.6-3.0 M NaCl. Immediately prior to data collection, crystals were harvested and soaked briefly in cryoprotectant solution, comprising 20% glycerol and the reservoir solution, Vapor diffusion, hanging drop, temperature 297K
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: Australian Synchrotron / ビームライン: MX1 / 波長: 0.95368 Å
検出器
タイプ: ADSC QUANTUM 210r / 検出器: CCD / 日付: 2010年9月1日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
解像度: 1.91→36.56 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.927 / WRfactor Rfree: 0.2358 / WRfactor Rwork: 0.1996 / Occupancy max: 1 / Occupancy min: 0.33 / FOM work R set: 0.8099 / SU B: 6.827 / SU ML: 0.106 / SU R Cruickshank DPI: 0.1693 / SU Rfree: 0.1537 / 交差検証法: THROUGHOUT / ESU R Free: 0.154 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES: WITH TLS ADDED
Rfactor
反射数
%反射
Selection details
Rfree
0.2453
4415
5 %
RANDOM
Rwork
0.2074
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all
0.2092
88211
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obs
0.2092
88211
98.9 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK