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- PDB-3sri: Crystal structure of Plasmodium falciparum AMA1 in complex with a... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3sri | ||||||
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Title | Crystal structure of Plasmodium falciparum AMA1 in complex with a 29aa PfRON2 peptide | ||||||
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![]() | CELL INVASION / AMA1 / Plasmodium falciparum / RON2 / malaria | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vulliez-Le Normand, B. / Saul, F.A. / Bentley, G.A. | ||||||
![]() | ![]() Title: Structural and functional insights into the malaria parasite moving junction complex. Authors: Vulliez-Le Normand, B. / Tonkin, M.L. / Lamarque, M.H. / Langer, S. / Hoos, S. / Roques, M. / Saul, F.A. / Faber, B.W. / Bentley, G.A. / Boulanger, M.J. / Lebrun, M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 85.4 KB | Display | ![]() |
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PDB format | ![]() | 61.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.2 KB | Display | ![]() |
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Full document | ![]() | 442.5 KB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Data in CIF | ![]() | 23.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3srjC ![]() 3zwzC ![]() 1z40S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43664.867 Da / Num. of mol.: 1 / Fragment: AMA1 / Mutation: N162K, T288V, S373D, N422D, S423K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: CAMP/Malaysia / Gene: AMA-1, PF83 / Production host: ![]() |
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#2: Protein/peptide | Mass: 3036.505 Da / Num. of mol.: 1 / Fragment: RON2 / Source method: obtained synthetically Source: (synth.) ![]() ![]() References: UniProt: Q8IKV6 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.36 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 8.6 Details: 20% PEG 4000, 0.1M Tris/HCL, 0.1M sodium acetate, 20% isopropanol, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Feb 28, 2011 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95367 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→46.97 Å / Num. all: 48207 / Num. obs: 48207 / % possible obs: 94.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.2 % / Biso Wilson estimate: 22.19 Å2 / Rmerge(I) obs: 0.056 / Net I/σ(I): 12.7 |
Reflection shell | Resolution: 1.6→1.69 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.618 / Mean I/σ(I) obs: 1.7 / Num. unique all: 6821 / % possible all: 92.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1Z40 Resolution: 1.6→35.04 Å / Cor.coef. Fo:Fc: 0.9517 / Cor.coef. Fo:Fc free: 0.9475 / SU R Cruickshank DPI: 0.078 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 29.98 Å2
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Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→35.04 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.6→1.64 Å / Total num. of bins used: 20
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