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- PDB-3ryw: Crystal structure of P. vivax geranylgeranyl diphosphate synthase... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ryw | ||||||
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Title | Crystal structure of P. vivax geranylgeranyl diphosphate synthase complexed with BPH-811 | ||||||
![]() | Farnesyl pyrophosphate synthase | ||||||
![]() | TRANSFERASE / geranylgeranyl diphosphate synthase / BPH-811 | ||||||
Function / homology | ![]() farnesyl diphosphate biosynthetic process / dimethylallyltranstransferase activity / geranyltranstransferase activity / membrane / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | No, J.H. / Liu, Y.-L. / Zhang, Y. / Oldfield, E. | ||||||
![]() | ![]() Title: Lipophilic analogs of zoledronate and risedronate inhibit Plasmodium geranylgeranyl diphosphate synthase (GGPPS) and exhibit potent antimalarial activity. Authors: No, J.H. / de Macedo Dossin, F. / Zhang, Y. / Liu, Y.L. / Zhu, W. / Feng, X. / Yoo, J.A. / Lee, E. / Wang, K. / Hui, R. / Freitas-Junior, L.H. / Oldfield, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 299.5 KB | Display | ![]() |
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PDB format | ![]() | 242.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3rbmC ![]() 3cc9S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46215.750 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: Salvador I / Gene: ggpps, PVX_092040 / Production host: ![]() ![]() #2: Chemical | ChemComp-K9H / { #3: Chemical | ChemComp-MG / #4: Chemical | ChemComp-SO4 / #5: Water | ChemComp-HOH / | Sequence details | AUTHORS STATE THAT THERE IS A DIFFERENCE BETWEEN THE DATABASE SEQUENCE AND THE SEQUENCE THE AUTHORS ...AUTHORS STATE THAT THERE IS A DIFFERENCE | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38.46 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 19% PEG 3350, 1mM BPH-811, 2mM MgCl2, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Apr 5, 2010 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. all: 37105 / Num. obs: 36585 / % possible obs: 98.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5 % / Rmerge(I) obs: 0.141 |
Reflection shell | Resolution: 2.8→2.85 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.659 / Mean I/σ(I) obs: 1.05 / % possible all: 98.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 3CC9 Resolution: 2.9→35.22 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.902 / SU B: 18.411 / SU ML: 0.349 / Cross valid method: THROUGHOUT / ESU R Free: 0.472 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.868 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→35.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→2.975 Å / Total num. of bins used: 20
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