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- PDB-3r0a: Possible transcriptional regulator from Methanosarcina mazei Go1 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3r0a | ||||||
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Title | Possible transcriptional regulator from Methanosarcina mazei Go1 (gi 21227196) | ||||||
![]() | Putative transcriptional regulator | ||||||
![]() | Transcription regulator / Structural Genomics / PSI-Biology / Midwest Center for Structural Genomics / MCSG / possible transcriptional regulator | ||||||
Function / homology | Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain / Arc Repressor Mutant, subunit A / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / Orthogonal Bundle / Mainly Alpha / Putative transcriptional regulator![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Joachimiak, A. / Duke, N.E.C. / Li, H. / Gu, M. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: Possible transcriptional regulator from Methanosarcina mazei Go1 (gi 21227196) Authors: Joachimiak, A. / Duke, N.E.C. / Li, H. / Gu, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57 KB | Display | ![]() |
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PDB format | ![]() | 46.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.6 KB | Display | ![]() |
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Full document | ![]() | 442.1 KB | Display | |
Data in XML | ![]() | 11.8 KB | Display | |
Data in CIF | ![]() | 15.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14337.048 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.33 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6 Details: Index screen, conditions #92 (H-8), 0.1M magnesium formate dihydrate, 15% w/v polyethylene glycol 3350, pH approximately 6.0 cryoprotected using Paratone-N, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Aug 25, 2010 Details: double crystal Si(111) monochromator, sagitally bent mirror |
Radiation | Monochromator: double crystal Si(111) monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97934 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. all: 11225 / Num. obs: 8825 / % possible obs: 78.6 % / Observed criterion σ(I): 3.8 / Redundancy: 9.8 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.191 Å2
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Refinement step | Cycle: LAST / Resolution: 2.31→49.32 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.31→2.37 Å / Total num. of bins used: 20
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