温度: 297 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 詳細: 20 mg/mL protein (in 10 mM BTP pH 7.5) mixed 1:1 with reservoir liquor containing 100 mM NaOAc (pH 4.6) and 0.6-3.0 M NaCl. Immediately prior to data collection, crystals were harvested and ...詳細: 20 mg/mL protein (in 10 mM BTP pH 7.5) mixed 1:1 with reservoir liquor containing 100 mM NaOAc (pH 4.6) and 0.6-3.0 M NaCl. Immediately prior to data collection, crystals were harvested and soaked briefly in cryoprotectant solution, comprising 20% glycerol and the reservoir solution, Vapor diffusion, hanging drop, temperature 297K
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データ収集
回折
平均測定温度: 120 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU MICROMAX-007 / 波長: 1.5418 Å
解像度: 1.9→32.76 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.939 / WRfactor Rfree: 0.2303 / WRfactor Rwork: 0.1891 / Occupancy max: 1 / Occupancy min: 0.5 / FOM work R set: 0.8558 / SU B: 7.076 / SU ML: 0.094 / SU R Cruickshank DPI: 0.1434 / SU Rfree: 0.1365 / 交差検証法: THROUGHOUT / ESU R Free: 0.137 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: WITH TLS ADDED
Rfactor
反射数
%反射
Selection details
Rfree
0.2285
4579
5 %
RANDOM
Rwork
0.1887
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all
0.1906
91174
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obs
0.1906
91174
99.38 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK