negative regulation of protein refolding / histone mRNA metabolic process / regulation of ATP-dependent activity / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / histone pre-mRNA 3'end processing complex / import into nucleus / SLBP independent Processing of Histone Pre-mRNAs ...negative regulation of protein refolding / histone mRNA metabolic process / regulation of ATP-dependent activity / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / histone pre-mRNA 3'end processing complex / import into nucleus / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / protein methylation / U12-type spliceosomal complex / methylosome / 7-methylguanosine cap hypermethylation / U1 snRNP binding / pICln-Sm protein complex / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / telomerase holoenzyme complex / negative regulation of chaperone-mediated autophagy / U2-type spliceosomal complex / U2-type precatalytic spliceosome / commitment complex / telomerase RNA binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / positive regulation of mRNA splicing, via spliceosome / RNA Polymerase II Transcription Termination / U1 snRNP / U2-type prespliceosome / termination of RNA polymerase II transcription / precatalytic spliceosome / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / regulation of RNA splicing / U5 snRNP / spliceosomal snRNP assembly / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / snRNP Assembly / SARS-CoV-2 modulates host translation machinery / nuclear body / nuclear speck / ribonucleoprotein complex / mRNA binding / enzyme binding / DNA binding / RNA binding / extracellular exosome / nucleoplasm / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function
snRNP70, RNA recognition motif / U1 small nuclear ribonucleoprotein of 70kDa N-terminal / U1 small nuclear ribonucleoprotein of 70kDa MW N terminal / U1 small nuclear ribonucleoprotein A, RNA recognition motif 2 / U1 small nuclear ribonucleoprotein A, RNA recognition motif 1 / Small ribonucleoprotein associated, SmB/SmN / Small nuclear ribonucleoprotein D1 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E ...snRNP70, RNA recognition motif / U1 small nuclear ribonucleoprotein of 70kDa N-terminal / U1 small nuclear ribonucleoprotein of 70kDa MW N terminal / U1 small nuclear ribonucleoprotein A, RNA recognition motif 2 / U1 small nuclear ribonucleoprotein A, RNA recognition motif 1 / Small ribonucleoprotein associated, SmB/SmN / Small nuclear ribonucleoprotein D1 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / : / Sm domain profile. / LSM domain superfamily / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily Similarity search - Domain/homology
DNA / RNA / RNA (> 10) / RNA (> 100) / Small nuclear ribonucleoprotein G / U1 small nuclear ribonucleoprotein 70 kDa / U1 small nuclear ribonucleoprotein A / Small nuclear ribonucleoprotein-associated proteins B and B' / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F ...DNA / RNA / RNA (> 10) / RNA (> 100) / Small nuclear ribonucleoprotein G / U1 small nuclear ribonucleoprotein 70 kDa / U1 small nuclear ribonucleoprotein A / Small nuclear ribonucleoprotein-associated proteins B and B' / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein-associated protein Similarity search - Component
biological assembly 1: chains A, S, Z, B, X, Y, F, E, G, R, D / biological assembly 2: chains P, L ,W, Q, U, V, I, H, J, N, M
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Components
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Protein , 9 types, 18 molecules APSLZWBQXUYVFIEHGJ
#1: Protein
U1-A / U1 snRNP A / U1 small nuclear ribonucleoprotein A / U1A / Coordinate model: Cα atoms only
Mass: 31319.533 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P09012
Mass: 51683.184 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P08621
#3: Protein
Sm-D3 / Small nuclear ribonucleoprotein Sm D3 / snRNP core protein D3 / Coordinate model: Cα atoms only
Mass: 13940.308 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P62318
#4: Protein
SmB / Small nuclear ribonucleoprotein polypeptides B and B1 / Coordinate model: Cα atoms only
Mass: 23686.004 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: Q66K91, UniProt: P14678*PLUS
#5: Protein
Sm-D1 / Small nuclear ribonucleoprotein Sm D1 / Sm-D autoantigen / snRNP core protein D1 / Coordinate model: Cα atoms only
Mass: 13310.653 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P62314
#6: Protein
Sm-D2 / Small nuclear ribonucleoprotein Sm D2 / snRNP core protein D2 / Coordinate model: Cα atoms only
Mass: 13551.928 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P62316
#7: Protein
Sm-F / snRNP-F / Sm protein F / Small nuclear ribonucleoprotein F / SmF / Coordinate model: Cα atoms only
Mass: 9734.171 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P62306
#8: Protein
Sm-E / snRNP-E / Sm protein E / Small nuclear ribonucleoprotein E / SmE / Coordinate model: Cα atoms only
Mass: 10817.601 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: P62304
#9: Protein
SmG / Small nuclear ribonucleoprotein polypeptide G / isoform CRA_a / Coordinate model: Cα atoms only
Mass: 8508.084 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: D6W5G6, UniProt: P62308*PLUS
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RNA chain / DNA chain , 2 types, 4 molecules RNDM
#10: RNA chain
U1snRNA / Coordinate model: P atoms only
Mass: 52697.070 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA
#11: DNA chain
DNA5'-D(*AP*GP*GP*TP*AP*AP*GP*TP*A)-3' / Coordinate model: P atoms only
Mass: 2803.873 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA oligonucleotide
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.39 Å3/Da / Density % sol: 48.43 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 8-10% PEG 4000, 0.1 M Trisodium citrate, 2 mM EDTA, 100 mM NaCl, 1-2% Anapoe X-305, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
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