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- PDB-3oun: Crystal structure of the FhaA FHA domain complexed with the intra... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3oun | ||||||
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Title | Crystal structure of the FhaA FHA domain complexed with the intracellular domain of Rv3910 | ||||||
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![]() | PROTEIN BINDING/TRANSFERASE / peptidoglycan / Ser/Thr kinase / pseudokinase / FHA domain / regulation / phosphorylation / membrane associated intracellular / PROTEIN BINDING-TRANSFERASE complex | ||||||
Function / homology | ![]() lipid-linked peptidoglycan transport / lipid-linked peptidoglycan transporter activity / lipid translocation / peptidoglycan biosynthetic process / peptidoglycan-based cell wall / regulation of cell shape / membrane => GO:0016020 / mRNA binding / extracellular region / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gee, C.L. / Alber, T. | ||||||
![]() | ![]() Title: A phosphorylated pseudokinase complex controls cell wall synthesis in mycobacteria Authors: Gee, C.L. / Papavinasasundaram, K.G. / Blair, S.R. / Baer, C.E. / Falick, A.M. / King, D.S. / Griffin, J.E. / Venghatakrishnan, H. / Zukauskas, A. / Wei, J.R. / Dhiman, R.K. / Crick, D.C. / ...Authors: Gee, C.L. / Papavinasasundaram, K.G. / Blair, S.R. / Baer, C.E. / Falick, A.M. / King, D.S. / Griffin, J.E. / Venghatakrishnan, H. / Zukauskas, A. / Wei, J.R. / Dhiman, R.K. / Crick, D.C. / Rubin, E.J. / Sassetti, C.M. / Alber, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.8 KB | Display | ![]() |
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PDB format | ![]() | 62.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435.2 KB | Display | ![]() |
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Full document | ![]() | 437.4 KB | Display | |
Data in XML | ![]() | 16.4 KB | Display | |
Data in CIF | ![]() | 23.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3otvSC ![]() 3oukC ![]() 3uqcC ![]() 2fezS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 16687.986 Da / Num. of mol.: 1 / Fragment: UNP residues 390-524 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 30481.244 Da / Num. of mol.: 1 / Fragment: UNP residues 676-963 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Chemical | ChemComp-MN / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.87 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.2M CaCl2, 0.1M MES pH 6, 1mM MnCl2, 20% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 10, 2009 / Details: DCM |
Radiation | Monochromator: DCM / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.115872 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. all: 14950 / Num. obs: 14926 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.7 % / Biso Wilson estimate: 36.8 Å2 / Rsym value: 0.23 / Net I/σ(I): 6.3 |
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 6.8 % / Mean I/σ(I) obs: 1.77 / Num. unique all: 1467 / Rsym value: 0.904 / % possible all: 99.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 3OTV and 2FEZ Resolution: 2.705→49.431 Å / SU ML: 0.35 / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 1.34 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 21.856 Å2 / ksol: 0.352 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.705→49.431 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 5
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