- PDB-3oq9: Structure of the FAS/FADD death domain assembly -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 3oq9
Title
Structure of the FAS/FADD death domain assembly
Components
Protein FADD
Tumor necrosis factor receptor superfamily member 6
Keywords
APOPTOSIS / DISC / FAS / FADD
Function / homology
Function and homology information
FasL/ CD95L signaling / dendrite regeneration / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / inflammatory cell apoptotic process / positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation / negative regulation of activation-induced cell death of T cells / regulation of myeloid cell differentiation / negative regulation of B cell activation ...FasL/ CD95L signaling / dendrite regeneration / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / inflammatory cell apoptotic process / positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation / negative regulation of activation-induced cell death of T cells / regulation of myeloid cell differentiation / negative regulation of B cell activation / death effector domain binding / regulation of B cell differentiation / FasL/ CD95L signaling / B cell mediated immunity / negative regulation of Schwann cell migration / tumor necrosis factor receptor superfamily binding / tumor necrosis factor receptor activity / lymphocyte apoptotic process / TRAIL signaling / CD95 death-inducing signaling complex / ripoptosome / TRAIL-activated apoptotic signaling pathway / death-inducing signaling complex assembly / Defective RIPK1-mediated regulated necrosis / TRIF-mediated programmed cell death / TLR3-mediated TICAM1-dependent programmed cell death / negative regulation of Schwann cell proliferation / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / positive regulation of adaptive immune response / Caspase activation via Death Receptors in the presence of ligand / regulation of hematopoietic progenitor cell differentiation / negative thymic T cell selection / positive regulation of macrophage differentiation / necroptotic signaling pathway / caspase binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / apical dendrite / death-inducing signaling complex / receptor serine/threonine kinase binding / negative regulation of necroptotic process / activation of cysteine-type endopeptidase activity / positive regulation of innate immune response / positive regulation of type I interferon-mediated signaling pathway / tumor necrosis factor receptor binding / death receptor binding / positive regulation of extrinsic apoptotic signaling pathway / motor neuron apoptotic process / TNFR1-induced proapoptotic signaling / regulation of T cell differentiation / RIPK1-mediated regulated necrosis / regulation of stress-activated MAPK cascade / cellular response to lithium ion / activation-induced cell death of T cells / hepatocyte apoptotic process / positive regulation of activated T cell proliferation / positive regulation of execution phase of apoptosis / T cell homeostasis / positive regulation of release of cytochrome c from mitochondria / positive regulation of proteolysis / behavioral response to cocaine / extrinsic apoptotic signaling pathway via death domain receptors / lymph node development / signaling adaptor activity / extrinsic apoptotic signaling pathway / response to glucocorticoid / extrinsic apoptotic signaling pathway in absence of ligand / spleen development / thymus development / secretory granule / kidney development / positive regulation of interleukin-8 production / apoptotic signaling pathway / Regulation of TNFR1 signaling / positive regulation of protein-containing complex assembly / Regulation of necroptotic cell death / cytoplasmic side of plasma membrane / response to toxic substance / sarcolemma / circadian rhythm / cellular response to mechanical stimulus / positive regulation of T cell mediated cytotoxicity / positive regulation of tumor necrosis factor production / positive regulation of type II interferon production / positive regulation of neuron apoptotic process / regulation of cell population proliferation / gene expression / cell body / T cell differentiation in thymus / regulation of apoptotic process / neuron apoptotic process / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / protease binding / molecular adaptor activity / calmodulin binding / positive regulation of apoptotic process / membrane raft / apical plasma membrane Similarity search - Function
Fas receptor / Fas receptor, death domain / Fas receptor, N-terminal / : / FADD / Death effector domain / Death effector domain / Death effector domain (DED) profile. / Death effector domain / TNFR/NGFR family cysteine-rich region domain profile. ...Fas receptor / Fas receptor, death domain / Fas receptor, N-terminal / : / FADD / Death effector domain / Death effector domain / Death effector domain (DED) profile. / Death effector domain / TNFR/NGFR family cysteine-rich region domain profile. / TNFR/NGFR cysteine-rich region / TNFR/NGFR family cysteine-rich region signature. / Tumor necrosis factor receptor / nerve growth factor receptor repeats. / TNFR/NGFR cysteine-rich region / Death domain profile. / DEATH domain, found in proteins involved in cell death (apoptosis). / Death domain / Death domain / Death-like domain superfamily Similarity search - Domain/homology
Tumor necrosis factor receptor superfamily member 6 / FAS-associated death domain protein Similarity search - Component
Mass: 10162.680 Da / Num. of mol.: 5 / Fragment: UNP residues 223-308 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Fas, Apt1, Tnfrsf6 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P25446
#2: Protein
ProteinFADD / FAS-associated death domain protein / FAS-associating death domain-containing protein / Mediator of ...FAS-associated death domain protein / FAS-associating death domain-containing protein / Mediator of receptor induced toxicity / Growth-inhibiting gene 3 protein
Mass: 11685.176 Da / Num. of mol.: 5 / Fragment: UNP residues 93-184 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FADD, MORT1, GIG3 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q13158
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.1 Å3/Da / Density % sol: 60 %
Crystal grow
Temperature: 298 K / Method: hanging drop / pH: 8.5 Details: 0.1 M Tris pH 8.5, 100 mM MgCl2, 5 % glycerol and 6-10 % PEG4000, hanging drop, temperature 298K
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