- PDB-3oit: Crystal structure of curcuminoid synthase CUS from Oryza sativa -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 3oit
Title
Crystal structure of curcuminoid synthase CUS from Oryza sativa
Components
Os07g0271500 protein
Keywords
TRANSFERASE / type III polyketide synthases
Function / homology
Function and homology information
bisdemethoxycurcumin synthase / bisdemethoxycurcumin synthase activity / flavonoid biosynthetic process / polyketide biosynthetic process / acyltransferase activity, transferring groups other than amino-acyl groups / identical protein binding Similarity search - Function
Chalcone/stilbene synthase, N-terminal / Polyketide synthase, type III / Chalcone/stilbene synthase, C-terminal / Chalcone and stilbene synthases, C-terminal domain / Chalcone and stilbene synthases, N-terminal domain / Thiolase/Chalcone synthase / Peroxisomal Thiolase; Chain A, domain 1 / Thiolase-like / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 438 / Source method: isolated from a natural source / Formula: H2O
Sequence details
CDNA WITH ACCESSION NUMBER AK109558 WAS USED FOR THE PRODUCTION OF PROTEIN CUS. AND AUTHOR STATED ...CDNA WITH ACCESSION NUMBER AK109558 WAS USED FOR THE PRODUCTION OF PROTEIN CUS. AND AUTHOR STATED RESIDUE 46 SHOULD BE ILE IN THE SEQUENCE OF AK109558.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.51 Å3/Da / Density % sol: 50.92 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 100mM Tris-HCl, 20% PEG 4000, 200mM NaCl, 6% 1,6-hexanediol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
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