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- PDB-3oiq: Crystal structure of yeast telomere protein Cdc13 OB1 and the cat... -
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Basic information
Entry | Database: PDB / ID: 3oiq | ||||||
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Title | Crystal structure of yeast telomere protein Cdc13 OB1 and the catalytic subunit of DNA polymerase alpha Pol1 | ||||||
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![]() | PROTEIN BINDING / OB fold / dimer / dimeric complex | ||||||
Function / homology | ![]() Inhibition of replication initiation of damaged DNA by RB1/E2F1 / CST complex / ribonucleoprotein complex localization / RNA-templated DNA biosynthetic process / DNA replication initiation / telomerase inhibitor activity / translation elongation factor binding / Processive synthesis on the lagging strand / Removal of the Flap Intermediate / Polymerase switching ...Inhibition of replication initiation of damaged DNA by RB1/E2F1 / CST complex / ribonucleoprotein complex localization / RNA-templated DNA biosynthetic process / DNA replication initiation / telomerase inhibitor activity / translation elongation factor binding / Processive synthesis on the lagging strand / Removal of the Flap Intermediate / Polymerase switching / premeiotic DNA replication / alpha DNA polymerase:primase complex / regulation of telomere maintenance via telomerase / Activation of the pre-replicative complex / nuclear telomere cap complex / single-stranded telomeric DNA binding / lagging strand elongation / G-rich strand telomeric DNA binding / telomere capping / mitotic DNA replication initiation / DNA synthesis involved in DNA repair / leading strand elongation / DNA replication origin binding / telomere maintenance via telomerase / DNA replication initiation / telomere maintenance / replication fork / double-strand break repair / single-stranded DNA binding / 4 iron, 4 sulfur cluster binding / DNA replication / chromosome, telomeric region / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / cell cycle / cell division / nucleotide binding / chromatin binding / mitochondrion / identical protein binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sun, J. / Yang, Y. / Wan, K. / Mao, N. / Yu, T.Y. / Lin, Y.C. / DeZwaan, D.C. / Freeman, B.C. / Lin, J.J. / Lue, N.F. / Lei, M. | ||||||
![]() | ![]() Title: Structural bases of dimerization of yeast telomere protein Cdc13 and its interaction with the catalytic subunit of DNA polymerase alpha. Authors: Sun, J. / Yang, Y. / Wan, K. / Mao, N. / Yu, T.Y. / Lin, Y.C. / DeZwaan, D.C. / Freeman, B.C. / Lin, J.J. / Lue, N.F. / Lei, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.4 KB | Display | ![]() |
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PDB format | ![]() | 40.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 434.9 KB | Display | ![]() |
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Full document | ![]() | 437.5 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 13.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3oipSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26596.436 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: CDC13, YDL220C / Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 4116.635 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.47 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 23% PEG3350, 0.2M Magnisium formate, 0.1M Tris-HCl, 5mM DTT, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Apr 22, 2010 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0782 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→100 Å / Num. all: 9997 / Num. obs: 9731 / % possible obs: 97.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3OIP Resolution: 2.4→50 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.4→50 Å
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Refine LS restraints |
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