THE FULL SEQUENCE OF THIS PROTEIN USED FOR CRYSTALLIZATION IS (MSE)GSSHHHHHHSSGVRGSH(MSE) ...THE FULL SEQUENCE OF THIS PROTEIN USED FOR CRYSTALLIZATION IS (MSE)GSSHHHHHHSSGVRGSH(MSE)KLSDIARLAGVSVTTASYVINGKAEQQRISNST VERVRAVVEAHGFTPNPQAAGLRSRHTRTLGFILPDLENPSYARIAKQLEQGARARGYQL LIASSDDQPDSERQLQQLFRARRCDALFVASCLPPEDDSYRELQDKGLPVIAIDRRLDPA HFCSVISDDRDASRQLAASLLSSAPRSIALIGARPELSVSQARAGGFDEALQGYTGEVRR YQGEAFSRECGQRL(MSE)QQLIDDLGGLPDALVTTSYVLLQGVFDTLQARPVDSRQLQL GTFGDNQLLDFLPLPVNA(MSE)AQQHGQIAATALELALAAIEEKRYEPGVHAVGRTFKQ RISVA. HOWEVER, RESIDUES AT N-TERMINUS SEEM TO BE DEGRADED IN CRYSTAL AND THE ACTUAL CRYSTALLIZED PROTEIN SEQUENCE MAY BE SHORTER. IN FACT MASS SPECTROMETRY COULD ONLY SHOW A PEAK OF 30.4KDA WEIGHT. ONLY THE ORDERED PART OF THE PROTEIN BY THE ELECTRON DENSITY MAP IS REPORTED IN SEQRES RECORDS. THE FIRST 18 RESIDUES (MSE)GSSHHHHHHSSGVRGSH ARE EXPRESSION TAG SEQUENCE.
構造決定の手法: 単波長異常分散 / 解像度: 2→14.928 Å / Occupancy max: 1 / Occupancy min: 0.36 / FOM work R set: 0.844 / SU ML: 0.27 / σ(F): 0.02 / 立体化学のターゲット値: ML / 詳細: THE FRIEDEL PAIRS WERE USED FOR PHASING.
Rfactor
反射数
%反射
Rfree
0.2179
3320
5.02 %
Rwork
0.1838
-
-
obs
0.1856
66122
96.92 %
溶媒の処理
減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL / Bsol: 43.413 Å2 / ksol: 0.383 e/Å3