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Yorodumi- PDB-3o6b: A Dual E3 Mechanism for Rub1 Ligation to Cdc53: Dcn1(P)-Cdc53(WHB... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3o6b | ||||||
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Title | A Dual E3 Mechanism for Rub1 Ligation to Cdc53: Dcn1(P)-Cdc53(WHB) low resolution | ||||||
Components |
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Keywords | Ligase / Cell Cycle | ||||||
Function / homology | Function and homology information Iron uptake and transport / regulation of transcription by galactose / regulation of sulfur amino acid metabolic process / cellular response to methylmercury / ubiquitin-like protein binding / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Antigen processing: Ubiquitination & Proteasome degradation / regulation of metabolic process / positive regulation of glucose transmembrane transport / protein neddylation ...Iron uptake and transport / regulation of transcription by galactose / regulation of sulfur amino acid metabolic process / cellular response to methylmercury / ubiquitin-like protein binding / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Antigen processing: Ubiquitination & Proteasome degradation / regulation of metabolic process / positive regulation of glucose transmembrane transport / protein neddylation / ubiquitin conjugating enzyme binding / mitotic intra-S DNA damage checkpoint signaling / silent mating-type cassette heterochromatin formation / mitochondrial fusion / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / SCF ubiquitin ligase complex / DNA replication origin binding / cullin family protein binding / subtelomeric heterochromatin formation / ubiquitin ligase complex / regulation of mitotic cell cycle / G1/S transition of mitotic cell cycle / G2/M transition of mitotic cell cycle / protein-macromolecule adaptor activity / ubiquitin-dependent protein catabolic process / chromosome, telomeric region / protein ubiquitination / cell division / ubiquitin protein ligase binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Scott, D.C. / Monda, J.K. / Grace, C.R.R. / Duda, D.M. / Kriwacki, R.W. / Kurz, T. / Schulman, B.A. | ||||||
Citation | Journal: Mol.Cell / Year: 2010 Title: A dual E3 mechanism for Rub1 ligation to Cdc53. Authors: Scott, D.C. / Monda, J.K. / Grace, C.R. / Duda, D.M. / Kriwacki, R.W. / Kurz, T. / Schulman, B.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3o6b.cif.gz | 263.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3o6b.ent.gz | 217 KB | Display | PDB format |
PDBx/mmJSON format | 3o6b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/o6/3o6b ftp://data.pdbj.org/pub/pdb/validation_reports/o6/3o6b | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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5 |
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Unit cell |
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-Components
#1: Protein | Mass: 24353.648 Da / Num. of mol.: 5 / Fragment: DCUN1 domain, residues 70-269 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: DCN1, YLR128W, L3111 / Production host: Escherichia coli (E. coli) / References: UniProt: Q12395 #2: Protein | Mass: 8652.943 Da / Num. of mol.: 5 / Fragment: residues 742-815 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: CDC53, YDL132W, D2190 / Production host: Escherichia coli (E. coli) / References: UniProt: Q12018 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.43 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.9 Details: 21% PEG 3350, 0.1M Bis-Tris-Propane, 0.2M NaF, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 77 K | |||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97924 Å | |||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 5, 2009 | |||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97924 Å / Relative weight: 1 | |||||||||||||||||||||
Reflection | Resolution: 3.1→40 Å / Num. all: 30487 / Num. obs: 30204 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 | |||||||||||||||||||||
Reflection shell |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.1→40 Å / σ(F): 0 / Stereochemistry target values: energy minimization
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Refinement step | Cycle: LAST / Resolution: 3.1→40 Å
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