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Open data
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Basic information
Entry | Database: PDB / ID: 3nnn | ||||||
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Title | BeF3 Activated DrrD Receiver Domain | ||||||
![]() | DNA BINDING RESPONSE REGULATOR D | ||||||
![]() | DNA BINDING PROTEIN / CheY-like fold / alpha/beta | ||||||
Function / homology | ![]() phosphorelay response regulator activity / protein-DNA complex / transcription cis-regulatory region binding / regulation of DNA-templated transcription / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Robinson, V.L. / Stock, A.M. | ||||||
![]() | ![]() Title: Regulation of response regulator autophosphorylation through interdomain contacts. Authors: Barbieri, C.M. / Mack, T.R. / Robinson, V.L. / Miller, M.T. / Stock, A.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.2 KB | Display | ![]() |
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PDB format | ![]() | 46.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.3 KB | Display | ![]() |
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Full document | ![]() | 443.5 KB | Display | |
Data in XML | ![]() | 12.9 KB | Display | |
Data in CIF | ![]() | 17.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3nhzC ![]() 3nnsC ![]() 1kgsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13992.295 Da / Num. of mol.: 2 / Fragment: N-terminal Domain (UNP residues 1-122) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.33 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.7 Details: 9-12 % PEG 3350, 0.2M Sodium phosphate monobasic, 6 mM beryllium chloride, 50 mM sodium fluoride, 10 mM magnesium chloride, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 31, 2004 |
Radiation | Monochromator: KOHZU double crystal monochromator with a sagitally focused second crystal. Crystal type Si(III) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. all: 14886 / Num. obs: 14831 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 5.9 % / Rmerge(I) obs: 0.087 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.41 / Mean I/σ(I) obs: 4.7 / Num. unique all: 1409 / Rsym value: 0.402 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1KGS Resolution: 2.2→30 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refine LS restraints |
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