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- PDB-3mi2: Crystal structure of human orotidine-5'-monophosphate decarboxyla... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3mi2 | ||||||
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Title | Crystal structure of human orotidine-5'-monophosphate decarboxylase complexed with pyrazofurin monophosphate | ||||||
![]() | Uridine 5'-monophosphate synthase | ||||||
![]() | LYASE / UMP synthase / Orotidine 5'-monophosphate Decarboxylase / pyrazofurin monophosphate | ||||||
Function / homology | ![]() UMP biosynthetic process / orotate phosphoribosyltransferase / orotate phosphoribosyltransferase activity / pyrimidine nucleobase biosynthetic process / Pyrimidine biosynthesis / orotidine-5'-phosphate decarboxylase / UDP biosynthetic process / orotidine-5'-phosphate decarboxylase activity / 'de novo' UMP biosynthetic process / 'de novo' pyrimidine nucleobase biosynthetic process ...UMP biosynthetic process / orotate phosphoribosyltransferase / orotate phosphoribosyltransferase activity / pyrimidine nucleobase biosynthetic process / Pyrimidine biosynthesis / orotidine-5'-phosphate decarboxylase / UDP biosynthetic process / orotidine-5'-phosphate decarboxylase activity / 'de novo' UMP biosynthetic process / 'de novo' pyrimidine nucleobase biosynthetic process / lactation / female pregnancy / cellular response to xenobiotic stimulus / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, Y. / To, T. / Kotra, L.P. / Pai, E.F. | ||||||
![]() | ![]() Title: Structural determinants for the inhibitory ligands of orotidine-5'-monophosphate decarboxylase. Authors: Meza-Avina, M.E. / Wei, L. / Liu, Y. / Poduch, E. / Bello, A.M. / Mishra, R.K. / Pai, E.F. / Kotra, L.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 136.9 KB | Display | ![]() |
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PDB format | ![]() | 105.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2p1fS ![]() 3bko S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30504.162 Da / Num. of mol.: 2 Fragment: Orotidine 5'-monophosphate Decarboxylase Domain (UNP residues 223-480) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P11172, orotidine-5'-phosphate decarboxylase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.75 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.4 Details: Ammonium Sulfate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jan 28, 2010 Details: DCM with cryo-cooled 1st crystal sagittally bent 2nd crystal followed by vertically focusing mirror |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 1.2→65.62 Å / Num. obs: 147972 / % possible obs: 84.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rmerge(I) obs: 0.044 / Rsym value: 0.044 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.2→1.24 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.288 / Mean I/σ(I) obs: 2.56 / Num. unique all: 8204 / Rsym value: 0.288 / % possible all: 46.9 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 2P1F Resolution: 1.2→65.62 Å / Cor.coef. Fo:Fc: 0.971 / Cor.coef. Fo:Fc free: 0.963 / SU B: 0.72 / SU ML: 0.032 / Cross valid method: THROUGHOUT / ESU R: 0.045 / ESU R Free: 0.047 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.886 Å2
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Refinement step | Cycle: LAST / Resolution: 1.2→65.62 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.2→1.232 Å / Total num. of bins used: 20
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