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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 3lzb | ||||||
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タイトル | EGFR kinase domain complexed with an imidazo[2,1-b]thiazole inhibitor | ||||||
![]() | Epidermal growth factor receptor | ||||||
![]() | TRANSFERASE/TRANSFERASE INHIBITOR / epidermal growth factor kinase domain / multitargeted small molecule kinase inhibitor / TRANSFERASE-TRANSFERASE INHIBITOR complex | ||||||
機能・相同性 | ![]() positive regulation of protein kinase C activity / multivesicular body, internal vesicle lumen / negative regulation of cardiocyte differentiation / response to hydroxyisoflavone / diterpenoid metabolic process / positive regulation of prolactin secretion / Shc-EGFR complex / Inhibition of Signaling by Overexpressed EGFR / ovulation cycle / EGFR interacts with phospholipase C-gamma ...positive regulation of protein kinase C activity / multivesicular body, internal vesicle lumen / negative regulation of cardiocyte differentiation / response to hydroxyisoflavone / diterpenoid metabolic process / positive regulation of prolactin secretion / Shc-EGFR complex / Inhibition of Signaling by Overexpressed EGFR / ovulation cycle / EGFR interacts with phospholipase C-gamma / positive regulation of mucus secretion / regulation of peptidyl-tyrosine phosphorylation / epidermal growth factor binding / tongue development / response to UV-A / PLCG1 events in ERBB2 signaling / midgut development / ERBB2-EGFR signaling pathway / hydrogen peroxide metabolic process / morphogenesis of an epithelial fold / PTK6 promotes HIF1A stabilization / ERBB2 Activates PTK6 Signaling / digestive tract morphogenesis / Signaling by EGFR / response to cobalamin / intracellular vesicle / negative regulation of epidermal growth factor receptor signaling pathway / eyelid development in camera-type eye / cerebral cortex cell migration / protein insertion into membrane / ERBB2 Regulates Cell Motility / protein tyrosine kinase activator activity / Respiratory syncytial virus (RSV) attachment and entry / positive regulation of bone resorption / Signaling by ERBB4 / PI3K events in ERBB2 signaling / negative regulation of mitotic cell cycle / positive regulation of phosphorylation / placental growth factor receptor activity / hepatocyte growth factor receptor activity / boss receptor activity / stem cell factor receptor activity / platelet-derived growth factor beta-receptor activity / brain-derived neurotrophic factor receptor activity / positive regulation of peptidyl-serine phosphorylation / platelet-derived growth factor alpha-receptor activity / epidermal growth factor receptor activity / hair follicle development / macrophage colony-stimulating factor receptor activity / peptidyl-tyrosine autophosphorylation / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / protein tyrosine kinase collagen receptor activity / MAP kinase kinase kinase activity / positive regulation of G1/S transition of mitotic cell cycle / GAB1 signalosome / transmembrane-ephrin receptor activity / insulin-like growth factor receptor activity / embryonic placenta development / vascular endothelial growth factor receptor activity / GPI-linked ephrin receptor activity / salivary gland morphogenesis / positive regulation of vasoconstriction / fibroblast growth factor receptor activity / insulin receptor activity / Signaling by ERBB2 / positive regulation of glial cell proliferation / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / transmembrane receptor protein tyrosine kinase activity / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / EGFR Transactivation by Gastrin / cellular response to dexamethasone stimulus / GRB2 events in ERBB2 signaling / positive regulation of synaptic transmission, glutamatergic / SHC1 events in ERBB2 signaling / ossification / basal plasma membrane / positive regulation of DNA repair / cellular response to epidermal growth factor stimulus / positive regulation of epithelial cell proliferation / positive regulation of superoxide anion generation / positive regulation of DNA replication / epithelial cell proliferation / neuron projection morphogenesis / Signal transduction by L1 / positive regulation of smooth muscle cell proliferation / positive regulation of protein localization to plasma membrane / astrocyte activation / liver regeneration / cellular response to amino acid stimulus / NOTCH3 Activation and Transmission of Signal to the Nucleus / cellular response to estradiol stimulus / phosphatidylinositol 3-kinase/protein kinase B signal transduction / lung development / EGFR downregulation / synaptic membrane / clathrin-coated endocytic vesicle membrane / peptidyl-tyrosine phosphorylation / Signaling by ERBB2 TMD/JMD mutants / Constitutive Signaling by EGFRvIII 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Swinger, K.K. | ||||||
![]() | ![]() タイトル: Imidazo[2,1-b]thiazoles: multitargeted inhibitors of both the insulin-like growth factor receptor and members of the epidermal growth factor family of receptor tyrosine kinases. 著者: Fidanze, S.D. / Erickson, S.A. / Wang, G.T. / Mantei, R. / Clark, R.F. / Sorensen, B.K. / Bamaung, N.Y. / Kovar, P. / Johnson, E.F. / Swinger, K.K. / Stewart, K.D. / Zhang, Q. / Tucker, L.A. ...著者: Fidanze, S.D. / Erickson, S.A. / Wang, G.T. / Mantei, R. / Clark, R.F. / Sorensen, B.K. / Bamaung, N.Y. / Kovar, P. / Johnson, E.F. / Swinger, K.K. / Stewart, K.D. / Zhang, Q. / Tucker, L.A. / Pappano, W.N. / Wilsbacher, J.L. / Wang, J. / Sheppard, G.S. / Bell, R.L. / Davidsen, S.K. / Hubbard, R.D. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 248.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 189.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.4 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.5 MB | 表示 | |
XML形式データ | ![]() | 48.3 KB | 表示 | |
CIF形式データ | ![]() | 64.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 2gs7S S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 36137.484 Da / 分子数: 8 / 断片: UNP residues 696-1022 / 変異: V924R / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 株 (発現宿主): Hi5 / 参照: UniProt: P00533, receptor protein-tyrosine kinase #2: 化合物 | ChemComp-ITI / #3: 水 | ChemComp-HOH / | 配列の詳細 | THE ACTUAL CRYSTALLIZED SEQUENCE FOR THE LAST 39 C-TERMINAL RESIDUES IS ...THE ACTUAL CRYSTALLIZ | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶化 | 温度: 290 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.7 詳細: 0.15M cesium chloride, 15% w/v polyethylene glycol 3350, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
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-データ収集
回折 | 平均測定温度: 77 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC QUANTUM 210 / 検出器: CCD / 日付: 2009年6月3日 / 詳細: mirrors |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1 Å / 相対比: 1 |
反射 | 解像度: 2.7→50 Å / Num. all: 36061 / Num. obs: 36046 / % possible obs: 99.8 % / 冗長度: 3.7 % / Biso Wilson estimate: 62.974 Å2 / Rmerge(I) obs: 0.131 / Rsym value: 0.131 / Net I/σ(I): 13.55 |
反射 シェル | 解像度: 2.7→2.8 Å / 冗長度: 3.6 % / Rmerge(I) obs: 0.52 / Mean I/σ(I) obs: 2.1 / Num. unique all: 3571 / Rsym value: 0.52 / % possible all: 76 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 2GS7 解像度: 2.7→44.17 Å / 交差検証法: THROUGHOUT / σ(F): 2.7 / σ(I): 2.7
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原子変位パラメータ | Biso mean: 57.9 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.7→44.17 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.7→2.8 Å / Total num. of bins used: 9
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