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Open data
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Basic information
Entry | Database: PDB / ID: 3lcg | ||||||
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Title | The D-sialic acid aldolase mutant V251L | ||||||
![]() | N-acetylneuraminate lyase | ||||||
![]() | LYASE / Tim barrel / Carbohydrate metabolism / Schiff base | ||||||
Function / homology | ![]() N-acetylneuraminate lyase / N-acetylneuraminate lyase activity / N-acetylneuraminate catabolic process / single-species biofilm formation / carbohydrate metabolic process / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chou, C.-Y. / Wang, A.H.-J. / Ko, T.-P. | ||||||
![]() | ![]() Title: Modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val251 Authors: Chou, C.-Y. / Ko, T.-P. / Wu, K.-J. / Huang, K.-F. / Lin, C.-H. / Wong, C.-H. / Wang, A.H.-J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 272.3 KB | Display | ![]() |
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PDB format | ![]() | 215.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 474.1 KB | Display | ![]() |
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Full document | ![]() | 484.8 KB | Display | |
Data in XML | ![]() | 59.5 KB | Display | |
Data in CIF | ![]() | 91.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3lbcC ![]() 3lbmC ![]() 3lcfC ![]() 3lchC ![]() 3lciC ![]() 3lclC ![]() 3lcwC ![]() 3lcxC ![]() 1nalS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 35305.316 Da / Num. of mol.: 4 / Mutation: V251L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.6 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 2.0M ammonium sulfate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Apr 23, 2009 / Details: vertically focusing mirror |
Radiation | Monochromator: horizontally focusing single crystal Si(111) bent Monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97622 Å / Relative weight: 1 |
Reflection | Resolution: 1.78→30 Å / Num. all: 161225 / Num. obs: 160580 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 1 / Redundancy: 6.6 % / Rmerge(I) obs: 0.046 / Net I/σ(I): 39.2 |
Reflection shell | Resolution: 1.78→1.84 Å / Redundancy: 6.4 % / Rmerge(I) obs: 0.628 / Mean I/σ(I) obs: 2.6 / Num. unique all: 15611 / % possible all: 98.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB enrty 1NAL Resolution: 1.78→28.01 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 74.418 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.834 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.78→28.01 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.78→1.84 Å / Rfactor Rfree error: 0.0121
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Xplor file |
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