ソフトウェア | 名称 | バージョン | 分類 |
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CrystalClear | | データ収集 | PHASER | | 位相決定 | REFMAC | 5.2.0019精密化 | d*TREK | | データ削減 | d*TREK | | データスケーリング | |
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精密化 | 構造決定の手法: 分子置換 開始モデル: pdb entry 3HGU 解像度: 2.8→26.78 Å / Cor.coef. Fo:Fc: 0.947 / Cor.coef. Fo:Fc free: 0.898 / SU B: 30.471 / SU ML: 0.273 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / ESU R: 0.583 / ESU R Free: 0.369 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
| Rfactor | 反射数 | %反射 | Selection details |
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Rfree | 0.2881 | 1495 | 5.1 % | RANDOM |
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Rwork | 0.21207 | - | - | - |
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obs | 0.21635 | 27888 | 99.89 % | - |
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK |
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原子変位パラメータ | Biso mean: 36.45 Å2 |
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精密化ステップ | サイクル: LAST / 解像度: 2.8→26.78 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 5538 | 0 | 40 | 16 | 5594 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | Dev ideal target | 数 |
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X-RAY DIFFRACTION | r_bond_refined_d0.03 | 0.022 | 5721 | X-RAY DIFFRACTION | r_angle_refined_deg2.713 | 1.963 | 7792 | X-RAY DIFFRACTION | r_dihedral_angle_1_deg9.767 | 5 | 704 | X-RAY DIFFRACTION | r_dihedral_angle_2_deg35.864 | 23.755 | 261 | X-RAY DIFFRACTION | r_dihedral_angle_3_deg22.246 | 15 | 935 | X-RAY DIFFRACTION | r_dihedral_angle_4_deg23.68 | 15 | 42 | X-RAY DIFFRACTION | r_chiral_restr0.176 | 0.2 | 867 | X-RAY DIFFRACTION | r_gen_planes_refined0.009 | 0.02 | 4392 | X-RAY DIFFRACTION | r_nbd_refined0.294 | 0.2 | 2818 | X-RAY DIFFRACTION | r_nbtor_refined0.349 | 0.2 | 3940 | X-RAY DIFFRACTION | r_xyhbond_nbd_refined0.196 | 0.2 | 223 | X-RAY DIFFRACTION | r_symmetry_vdw_refined0.235 | 0.2 | 33 | X-RAY DIFFRACTION | r_symmetry_hbond_refined0.191 | 0.2 | 3 | X-RAY DIFFRACTION | r_mcbond_it1.015 | 1.5 | 3612 | X-RAY DIFFRACTION | r_mcangle_it1.787 | 2 | 5714 | X-RAY DIFFRACTION | r_scbond_it2.697 | 3 | 2507 | X-RAY DIFFRACTION | r_scangle_it4.32 | 4.5 | 2077 | | | | | | | | | | | | | | | | | |
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LS精密化 シェル | 解像度: 2.8→2.877 Å / Total num. of bins used: 20 / | Rfactor | 反射数 |
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Rfree | 0.318 | 119 |
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Rwork | 0.25 | 2013 |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 34.3861 | -21.7153 | -8.0995 | 23.1187 | -11.3541 | 30.7459 | 0.949 | 0.0888 | 4.4171 | 0.8635 | -0.8247 | -1.4479 | 0.2759 | -1.4783 | -0.1243 | 0.1622 | -0.1286 | -0.0592 | 0.2746 | -0.1038 | 0.3653 | 51.192 | 36.559 | 55.837 | 2 | 20.3288 | -14.612 | -7.0804 | 18.9142 | -4.1658 | 12.6497 | 0.04 | -0.4379 | -1.2138 | 0.433 | 0.8184 | -1.2886 | -2.2518 | 1.7879 | -0.8583 | 0.3559 | -0.1174 | 0.0905 | 0.1853 | 0.0934 | -0.0117 | 51.825 | 32.52 | 64.134 | 3 | 6.5767 | -0.8015 | -5.6155 | 6.7798 | 15.4086 | 37.2397 | -2.1383 | -0.8853 | 0.0607 | 0.1341 | 2.0441 | -0.7389 | -0.503 | -0.2954 | 0.0942 | 0.657 | 0.1041 | -0.0232 | -0.0428 | 0.2469 | 0.3921 | 47.283 | 32.002 | 72.982 | 4 | 2.6948 | -0.977 | 0.1315 | 4.4145 | -2.1897 | 2.7194 | -0.0031 | 0.4103 | 0.7706 | -0.0729 | 0.2037 | -0.0468 | -1.1279 | -0.3103 | -0.2006 | 0.1867 | 0.1928 | -0.0026 | 0.2117 | 0.116 | 0.269 | 39.856 | 31.89 | 58.79 | 5 | 11.8997 | -3.2712 | -3.5906 | 3.436 | 0.415 | 1.2124 | 0.0806 | 0.2126 | 0.4773 | -0.2678 | -0.3606 | 0.4116 | -0.0961 | -1.0257 | 0.28 | 0.2506 | 0.2446 | -0.045 | 0.1689 | 0.0817 | 0.1725 | 31.057 | 30.855 | 62.497 | 6 | 4.8539 | -5.1059 | -2.0218 | 5.9463 | 3.7556 | 8.3303 | -0.0803 | 0.3043 | -0.3012 | -0.1293 | -0.8384 | 0.8602 | -0.3496 | -1.1738 | 0.9187 | 0.0151 | 0.1385 | -0.0136 | 0.3865 | 0.067 | 0.1856 | 32.496 | 19.24 | 58.518 | 7 | 1.8835 | 0.513 | 0.5646 | 16.9709 | 2.5217 | 9.9954 | -0.1458 | 0.1953 | -0.3104 | -0.384 | 0.2116 | -0.2027 | -0.7504 | 0.232 | -0.0658 | -0.0095 | 0.1718 | 0.0705 | 0.27 | 0.0797 | 0.0403 | 46.041 | 19.07 | 51.797 | 8 | 4.914 | -1.0065 | 2.7805 | 0.2703 | -1.0159 | 4.6811 | 0.0144 | -0.0298 | 0.1055 | -0.0757 | -0.123 | -0.1678 | -0.1994 | -0.2931 | 0.1087 | 0.306 | 0.1025 | 0.0904 | 0.1383 | 0.037 | 0.1532 | 49.143 | 15.275 | 60.879 | 9 | 15.6695 | -9.5507 | 1.7569 | 20.9989 | 17.7447 | 23.5224 | -1.2138 | -0.0232 | -0.4668 | 0.5988 | 1.6305 | -0.6684 | -0.5924 | 0.3104 | -0.4167 | 0.1103 | 0.1433 | 0.0525 | 0.208 | 0.1112 | 0.1588 | 66.405 | 13.71 | 74.435 | 10 | 0.0098 | -0.052 | -0.0925 | 1.3072 | -0.1213 | 1.2272 | 0.0036 | 0.0403 | 0.0821 | -0.0568 | -0.0859 | 0.1182 | -0.0876 | -0.1098 | 0.0823 | 0.1638 | 0.0293 | 0.0515 | 0.2662 | -0.0144 | 0.1982 | 55.711 | 4.118 | 59.887 | 11 | 35.4035 | -21.4442 | -9.8511 | 45.6088 | -11.9656 | 12.5994 | -0.8623 | 1.9744 | -0.5987 | 0.7853 | 2.1187 | 0.9364 | -0.1146 | -0.543 | -1.2564 | 0.1436 | -0.3494 | -0.0929 | 0.2772 | 0.1873 | 0.3175 | 38.424 | -10.719 | 59.752 | 12 | 6.5654 | 2.964 | 12.638 | 21.5213 | 10.9384 | 25.6839 | 1.119 | -0.1726 | -0.1104 | 2.2505 | -1.1294 | 1.2517 | 1.1083 | 0.199 | 0.0104 | 0.2294 | -0.137 | 0.1339 | 0.2405 | 0.0123 | 0.2413 | 49.3 | -13.35 | 59.536 | 13 | 3.3342 | 2.7266 | -1.4312 | 2.3232 | -0.7775 | 2.2643 | -0.0953 | -0.0322 | 0.0153 | 0.0962 | 0.0782 | -0.1041 | 0.3275 | -0.0742 | 0.0171 | 0.1846 | -0.0051 | 0.0347 | 0.2396 | 0.0014 | 0.2055 | 59.358 | -5.731 | 64.686 | 14 | 0.6086 | 1.2683 | -2.6748 | 2.6434 | -5.5746 | 11.7563 | -0.0997 | -0.1101 | -0.8052 | -0.8534 | 0.1544 | 0.2002 | 0.3398 | 0.3458 | -0.0546 | 0.2347 | -0.023 | 0.0687 | 0.1397 | -0.0252 | 0.2742 | 58.253 | -13.349 | 70.656 | 15 | 3.2983 | -0.5677 | -1.5892 | 2.155 | 0.4863 | 3.2535 | 0.0529 | -0.122 | 0.015 | 0.0481 | -0.0781 | -0.0572 | -0.0365 | -0.0359 | 0.0253 | 0.1256 | -0.0151 | 0.0378 | 0.2216 | -0.0058 | 0.1998 | 56.634 | -2.922 | 76.752 | 16 | 2.0299 | -1.0763 | 3.5458 | 1.3183 | -2.8518 | 7.4568 | 0.151 | 0.2595 | -0.1496 | 0.1169 | -0.0902 | 0.0056 | -0.2425 | -0.1242 | -0.0608 | 0.1793 | 0.1104 | 0.0318 | 0.2749 | -0.0386 | 0.2079 | 48.522 | 8.371 | 76.061 | 17 | 0.9106 | -0.6293 | 1.6893 | 1.9409 | -1.9195 | 3.5095 | 0.6864 | -0.5852 | 0.3631 | 0.1062 | -0.6217 | 0.4658 | -0.3563 | 0.5485 | -0.0647 | 0.2704 | 0.1623 | 0.0088 | 0.275 | -0.0514 | 0.2318 | 43.002 | 18.645 | 72.258 | 18 | 7.2657 | 0.3009 | -1.3781 | 1.2885 | -0.3127 | 0.3126 | 0.0135 | 0.1844 | -0.0178 | 0.2523 | -0.3753 | 0.3946 | -0.3552 | -0.8951 | 0.3618 | 0.2169 | 0.1827 | 0.0286 | 0.4495 | 0.0171 | 0.1489 | 30.85 | 13.414 | 79.712 | 19 | 4.3151 | 3.9512 | -1.3042 | 13.2518 | -7.7013 | 4.7894 | -0.1641 | 0.6529 | -0.0838 | -0.2131 | 0.1501 | -0.1577 | -0.0682 | -0.5328 | 0.014 | 0.139 | 0.1524 | 0.0193 | 0.3371 | -0.0333 | 0.1775 | 39.479 | 20.133 | 64.094 | 20 | 3.3185 | -0.927 | 2.2018 | 13.2798 | 3.0482 | 7.9311 | -0.1093 | 0.0623 | -0.0804 | 0.0913 | 0.177 | -0.2369 | -0.0216 | -0.6611 | -0.0677 | 0.043 | 0.0268 | 0.0508 | 0.2487 | 0.008 | 0.111 | 39.238 | 10.41 | 77.429 | 21 | 2.6661 | 2.2115 | 2.5751 | 2.8328 | 3.2723 | 4.6973 | -0.3285 | 0.0823 | 0.2959 | -0.29 | 0.0322 | 0.3043 | -0.9701 | -0.1937 | 0.2963 | 0.2744 | 0.0104 | 0.0072 | 0.1436 | 0.0532 | 0.2336 | 79.085 | 16.066 | 39.333 | 22 | 1.5038 | -0.512 | -0.1853 | 0.899 | -1.0948 | 1.873 | -0.0809 | -0.0461 | 0.0726 | -0.0443 | 0.0447 | 0.0745 | 0.0135 | 0.1636 | 0.0362 | 0.2385 | 0.0024 | 0.0093 | 0.2395 | 0.0078 | 0.1566 | 82.931 | 4.397 | 42.914 | 23 | 2.0648 | -0.9369 | -0.6252 | 0.4552 | 0.0329 | 2.2775 | 0.0732 | -0.1004 | -0.1934 | -0.1982 | -0.0856 | 0.1936 | 0.1397 | 0.0043 | 0.0125 | 0.248 | -0.0246 | 0.0248 | 0.1535 | 0.0117 | 0.1779 | 80.233 | -2.87 | 44.431 | 24 | 15.1412 | 3.0876 | 5.0253 | 8.8129 | 1.1524 | 1.6698 | 0.5077 | -1.0144 | 0.7624 | -0.0704 | -0.7797 | 0.7452 | -0.4552 | -0.3239 | 0.272 | 0.2416 | 0.0607 | 0.1629 | 0.2945 | 0.0293 | 0.0775 | 66.16 | 7.877 | 52.583 | 25 | 23.1871 | -36.6768 | -27.14 | 58.0146 | 42.9295 | 31.7668 | -3.897 | 1.4642 | -4.1005 | -2.6355 | -0.7987 | -1.312 | 2.7309 | -3.6498 | 4.6956 | 0.6057 | -0.3698 | 0.0783 | 0.5262 | -0.0945 | 0.5497 | 61.038 | -11.167 | 50.477 | 26 | 1.8143 | -0.8405 | 0.6708 | 2.1236 | -2.0285 | 1.9494 | -0.0368 | -0.0105 | 0.1317 | 0.0178 | -0.1604 | 0.0431 | -0.1276 | -0.4197 | 0.1972 | 0.2218 | -0.0068 | 0.0132 | 0.2552 | -0.0096 | 0.1389 | 64.327 | 7.826 | 41.797 | 27 | 4.0215 | -0.2254 | 4.6247 | 0.0126 | -0.2592 | 5.3184 | -1.1065 | 0.6767 | -0.7178 | 0.2092 | 0.3248 | 2.0494 | -2.2059 | 0.4111 | 0.7817 | 0.5364 | 0.1276 | -0.27 | 0.664 | 0.2463 | 0.1342 | 54.105 | 18.282 | 29.275 | 28 | 1.287 | -0.5391 | -0.5405 | 1.6864 | -1.3831 | 2.0006 | -0.0109 | 0.0563 | -0.0423 | 0.0715 | -0.0563 | -0.0971 | -0.1759 | -0.3919 | 0.0672 | 0.1398 | 0.0458 | -0.0564 | 0.3082 | -0.0128 | 0.1548 | 51.125 | 5.365 | 44.72 | 29 | 10.967 | 2.3113 | 2.7007 | 42.6947 | 7.31 | 4.5107 | -0.7917 | -0.1757 | -1.86 | 0.2325 | -0.0136 | 0.2704 | 0.7308 | 0.9389 | 0.8053 | 0.2215 | 0.038 | 0.0568 | 0.2878 | -0.2307 | 0.2539 | 49.842 | -17.443 | 48.201 | 30 | 2.7124 | -1.2347 | 2.1655 | 0.5726 | -0.8931 | 2.5477 | -0.002 | 0.5876 | -0.7543 | -0.1002 | 0.271 | 1.1507 | -0.1184 | -0.0356 | -0.269 | 0.0531 | -0.092 | -0.1582 | 0.4244 | -0.0406 | 0.3435 | 39.028 | -5.576 | 45.687 | 31 | 4.0704 | -1.4568 | -0.0159 | 7.1418 | 2.9957 | 1.3505 | -0.3777 | 0.1603 | 0.2764 | -0.1687 | 0.5827 | 0.2502 | 0.1109 | -0.4527 | -0.205 | 0.1339 | 0.0064 | -0.1086 | 0.4279 | 0.0777 | 0.1836 | 42.224 | 5.034 | 38.366 | 32 | 57.1766 | 16.3786 | -5.751 | 4.9456 | -3.2297 | 10.4395 | 0.5328 | 0.59 | -0.0788 | 0.2094 | -0.4993 | 2.4609 | 1.2226 | 0.4195 | -0.0335 | 0.0256 | 0.0636 | -0.1592 | 0.4227 | -0.1801 | 0.3589 | 36.512 | -4.735 | 35.984 | 33 | 7.6126 | -2.1884 | 6.3178 | 6.4196 | -3.8214 | 7.3375 | -0.0505 | -0.0828 | -0.0986 | -0.3016 | 0.3836 | 0.558 | -0.1393 | -0.5269 | -0.3331 | 0.0511 | 0.0797 | -0.1379 | 0.3725 | -0.0561 | 0.1634 | 39.777 | 4.345 | 33.481 | 34 | 11.1178 | 17.561 | 2.7845 | 39.4138 | 5.4079 | 0.7847 | 1.2402 | -0.4695 | -2.2622 | 2.1275 | -1.4115 | 0.3833 | 0.9216 | -0.7265 | 0.1713 | 0.1503 | -0.0627 | -0.1133 | 0.4085 | -0.1637 | 0.2465 | 47.418 | -6.626 | 26.488 | 35 | 2.5924 | 1.2486 | -1.6968 | 2.0797 | 0.3813 | 2.0825 | -0.1245 | 0.1442 | -0.1833 | -0.201 | 0.3862 | 0.2857 | -0.3857 | -0.2556 | -0.2617 | 0.1542 | 0.0401 | -0.1152 | 0.4037 | -0.0595 | 0.1173 | 45.821 | 1.449 | 27.718 | 36 | 2.4335 | 0.1858 | 4.7395 | 1.2726 | 0.1299 | 10.4348 | -0.1547 | 0.3092 | 0.218 | -0.1935 | -0.0519 | 0.3135 | -0.2292 | 0.1904 | 0.2066 | 0.206 | 0.0359 | -0.0245 | 0.3341 | -0.0462 | 0.1199 | 57.653 | 0.537 | 27.597 | 37 | 0.8077 | -0.3086 | -0.7323 | 4.2906 | 0.1175 | 0.9511 | 0.2432 | 0.2082 | -0.1981 | -0.2298 | -0.0873 | -0.0651 | 0.0067 | -0.0597 | -0.1559 | 0.2098 | 0.0272 | -0.0406 | 0.3211 | -0.023 | 0.1327 | 71.552 | -6.369 | 28.869 | 38 | 2.0727 | -0.7825 | -0.2191 | 0.4761 | -0.125 | 0.262 | 0.2703 | 0.0785 | -0.4973 | -0.2714 | -0.1509 | -0.3527 | -0.0234 | 0.286 | -0.1195 | 0.2591 | -0.0273 | -0.0517 | 0.301 | -0.0406 | 0.1984 | 68.374 | -8.402 | 30.874 | 39 | 3.1142 | 1.1483 | 3.0912 | 3.7173 | 2.0948 | 3.3452 | 0.0598 | 0.2222 | -0.0493 | -0.1057 | 0.0259 | 0.0787 | -0.1204 | 0.2411 | -0.0857 | 0.1368 | 0.0084 | 0.0252 | 0.2511 | 0.0079 | 0.1105 | 70.012 | -1.881 | 32.769 | 40 | 12.4633 | 0.1832 | -1.8196 | 10.2974 | -4.0326 | 9.9151 | 0.2388 | 0.2929 | -0.8372 | -1.3732 | 0.6267 | 1.8587 | 1.4956 | -0.0402 | -0.8655 | 0.2907 | 0.0015 | -0.1038 | 0.0978 | -0.1463 | 0.1885 | 59.624 | -12.085 | 30.424 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Auth asym-ID | Auth seq-ID |
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1 | X-RAY DIFFRACTION | 1 | A3 - 7 | 2 | X-RAY DIFFRACTION | 2 | A8 - 13 | 3 | X-RAY DIFFRACTION | 3 | A14 - 22 | 4 | X-RAY DIFFRACTION | 4 | A23 - 40 | 5 | X-RAY DIFFRACTION | 5 | A41 - 60 | 6 | X-RAY DIFFRACTION | 6 | A61 - 78 | 7 | X-RAY DIFFRACTION | 7 | A79 - 99 | 8 | X-RAY DIFFRACTION | 8 | A100 - 123 | 9 | X-RAY DIFFRACTION | 9 | A124 - 130 | 10 | X-RAY DIFFRACTION | 10 | A131 - 170 | 11 | X-RAY DIFFRACTION | 11 | A171 - 184 | 12 | X-RAY DIFFRACTION | 12 | A185 - 189 | 13 | X-RAY DIFFRACTION | 13 | A190 - 213 | 14 | X-RAY DIFFRACTION | 14 | A214 - 224 | 15 | X-RAY DIFFRACTION | 15 | A225 - 261 | 16 | X-RAY DIFFRACTION | 16 | A262 - 285 | 17 | X-RAY DIFFRACTION | 17 | A286 - 297 | 18 | X-RAY DIFFRACTION | 18 | A298 - 313 | 19 | X-RAY DIFFRACTION | 19 | A314 - 329 | 20 | X-RAY DIFFRACTION | 20 | A330 - 354 | 21 | X-RAY DIFFRACTION | 21 | B3 - 17 | 22 | X-RAY DIFFRACTION | 22 | B18 - 45 | 23 | X-RAY DIFFRACTION | 23 | B46 - 86 | 24 | X-RAY DIFFRACTION | 24 | B87 - 95 | 25 | X-RAY DIFFRACTION | 25 | B96 - 103 | 26 | X-RAY DIFFRACTION | 26 | B104 - 123 | 27 | X-RAY DIFFRACTION | 27 | B124 - 130 | 28 | X-RAY DIFFRACTION | 28 | B131 - 171 | 29 | X-RAY DIFFRACTION | 29 | B172 - 179 | 30 | X-RAY DIFFRACTION | 30 | B180 - 198 | 31 | X-RAY DIFFRACTION | 31 | B199 - 213 | 32 | X-RAY DIFFRACTION | 32 | B214 - 219 | 33 | X-RAY DIFFRACTION | 33 | B220 - 238 | 34 | X-RAY DIFFRACTION | 34 | B239 - 246 | 35 | X-RAY DIFFRACTION | 35 | B247 - 264 | 36 | X-RAY DIFFRACTION | 36 | B265 - 286 | 37 | X-RAY DIFFRACTION | 37 | B287 - 307 | 38 | X-RAY DIFFRACTION | 38 | B308 - 317 | 39 | X-RAY DIFFRACTION | 39 | B318 - 346 | 40 | X-RAY DIFFRACTION | 40 | B347 - 354 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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