+Open data
-Basic information
Entry | Database: PDB / ID: 3ksn | ||||||
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Title | Crystal structure of the lipoprotein localization factor, LolA | ||||||
Components | Outer-membrane lipoprotein carrier protein | ||||||
Keywords | PROTEIN TRANSPORT / lipoprotein carrier / Chaperone / Transport | ||||||
Function / homology | Function and homology information lipoprotein localization to outer membrane / lipoprotein transport / outer membrane-bounded periplasmic space Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.65 Å | ||||||
Authors | Ahmadpour, F. / Gloyd, M. / Guarne, A. / Stewart, G. / Pathania, R. / Brown, E.D. | ||||||
Citation | Journal: To be Published Title: Crystal structure of the lipoprotein localization factor, LolA Authors: Ahmadpour, F. / Gloyd, M. / Guarne, A. / Stewart, G. / Pathania, R. / Brown, E.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ksn.cif.gz | 85.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ksn.ent.gz | 64.5 KB | Display | PDB format |
PDBx/mmJSON format | 3ksn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ks/3ksn ftp://data.pdbj.org/pub/pdb/validation_reports/ks/3ksn | HTTPS FTP |
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-Related structure data
Related structure data | 1au8S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 20400.348 Da / Num. of mol.: 1 / Fragment: Residues 22-203 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: b0891, JW0874, lolA, lplA, yzzV / Plasmid: pDEST17 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P61316 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.78 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4.4 Details: 25% PEG 1000, 20% PEG 8000, 0.1 M sodium acetate, dehydrated over 1M ammonium sulfate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.0809 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 24, 2008 / Details: mirrors |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0809 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→50 Å / Num. obs: 20611 / % possible obs: 99.6 % / Redundancy: 9.8 % / Biso Wilson estimate: 23.11 Å2 / Rmerge(I) obs: 0.047 / Rsym value: 0.035 / Net I/σ(I): 39.5 |
Reflection shell | Resolution: 1.65→1.71 Å / Redundancy: 9.8 % / Rmerge(I) obs: 0.534 / Mean I/σ(I) obs: 4.47 / Num. unique all: 2035 / Rsym value: 0.491 / % possible all: 99.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1AU8 Resolution: 1.65→31.577 Å / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 1.89 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 60.497 Å2 / ksol: 0.4 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.65→31.577 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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