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- PDB-3kkl: Crystal structure of functionally unknown HSP33 from Saccharomyce... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3kkl | ||||||
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Title | Crystal structure of functionally unknown HSP33 from Saccharomyces cerevisiae | ||||||
![]() | Probable chaperone protein HSP33 | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hwang, K.Y. / Sung, M.W. / Lee, W.H. | ||||||
![]() | ![]() Title: Crystal structure of functionally unknown HSP33 from Saccharomyces cerevisiae Authors: Hwang, K.Y. / Sung, M.W. / Lee, W.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93.6 KB | Display | ![]() |
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PDB format | ![]() | 71.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1qvzS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26878.396 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: HSP33, YOR391C / Plasmid: pET22b / Production host: ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.81 Å3/Da / Density % sol: 32.18 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.2M Tri-sodium Citrate dihydrate, 23%(w/v) polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Sep 30, 2007 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.03→50 Å / Num. obs: 24915 / % possible obs: 92.5 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / Num. measured all: 109787 |
Reflection shell | Resolution: 2.03→2.1 Å / % possible all: 87.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1qvz Resolution: 2.03→19.76 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 38.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.03→19.76 Å
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Refine LS restraints |
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