+Open data
-Basic information
Entry | Database: PDB / ID: 3kf8 | ||||||
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Title | Crystal structure of C. tropicalis Stn1-ten1 complex | ||||||
Components |
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Keywords | STRUCTURAL PROTEIN / OB fold | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Candida tropicalis MYA-3404 (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.4 Å | ||||||
Authors | Sun, J. / Yu, E.Y. / Yang, Y.T. / Confer, L.A. / Sun, S.H. / Wan, K. / Lue, N.F. / Lei, M. | ||||||
Citation | Journal: Genes Dev. / Year: 2009 Title: Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres. Authors: Sun, J. / Yu, E.Y. / Yang, Y. / Confer, L.A. / Sun, S.H. / Wan, K. / Lue, N.F. / Lei, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3kf8.cif.gz | 143.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3kf8.ent.gz | 113.5 KB | Display | PDB format |
PDBx/mmJSON format | 3kf8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3kf8_validation.pdf.gz | 454.8 KB | Display | wwPDB validaton report |
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Full document | 3kf8_full_validation.pdf.gz | 475.9 KB | Display | |
Data in XML | 3kf8_validation.xml.gz | 28.1 KB | Display | |
Data in CIF | 3kf8_validation.cif.gz | 38.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kf/3kf8 ftp://data.pdbj.org/pub/pdb/validation_reports/kf/3kf8 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 26047.814 Da / Num. of mol.: 2 / Fragment: N-terminal fragment: UNP residues 2-217 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida tropicalis MYA-3404 (yeast) / Strain: ATCC MYA-3404 / T1 / Gene: CTRG_01841 / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: C5M7K9 #2: Protein | Mass: 13709.496 Da / Num. of mol.: 2 / Fragment: UNP residues 2-123 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida tropicalis MYA-3404 (yeast) / Strain: ATCC MYA-3404 / T1 / Gene: CTRG_00988 / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: C5M4J8 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.06 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 12% PEG 4000, 12% Isopropanol, 0.1 M Sodium citrate pH 5.6, 5 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 1 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Apr 14, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. all: 34781 / Num. obs: 34725 / % possible obs: 99.8 % / Redundancy: 11 % / Rmerge(I) obs: 0.065 / Net I/σ(I): 31.2 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.4→50 Å
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Refinement step | Cycle: LAST / Resolution: 2.4→50 Å
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