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Open data
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Basic information
Entry | Database: PDB / ID: 3io0 | ||||||
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Title | Crystal structure of EtuB from Clostridium kluyveri | ||||||
![]() | EtuB protein | ||||||
![]() | STRUCTURAL PROTEIN / Tamdem repeat of bacterial microcompartment domain in a single polypeptide chain | ||||||
Function / homology | Bacterial microcompartment shell protein PduB / BMC domain / Bacterial microcompartment domain / CcmK-like superfamily / BMC / structural molecule activity / Predicted microcompartment protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pickersgill, R.W. / Warren, M.J. | ||||||
![]() | ![]() Title: Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilisation in Clostridium kluyveri. Authors: Heldt, D. / Frank, S. / Seyedarabi, A. / Ladakis, D. / Parsons, J.B. / Warren, M.J. / Pickersgill, R.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 52.7 KB | Display | ![]() |
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PDB format | ![]() | 38.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.6 KB | Display | ![]() |
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Full document | ![]() | 429.8 KB | Display | |
Data in XML | ![]() | 10.7 KB | Display | |
Data in CIF | ![]() | 13.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23756.201 Da / Num. of mol.: 1 / Fragment: UNP residues 75-304 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC8527, DSM555, NCIMB 10680 / Gene: 1073 / Plasmid: pET14b / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 9.14 Å3/Da / Density % sol: 86.55 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 4M formate in water, 8mg/ml protein, equal volumes of reservoir and protein formed the drop, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 27, 2008 / Details: Si (111) |
Radiation | Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9762 Å / Relative weight: 1 |
Reflection | Resolution: 3→86.7 Å / Num. all: 17475 / Num. obs: 17475 / % possible obs: 100 % / Observed criterion σ(F): 187378 / Observed criterion σ(I): 187378 / Redundancy: 10.7 % / Biso Wilson estimate: 68.3 Å2 / Rmerge(I) obs: 0.151 / Net I/σ(I): 16.8 |
Reflection shell | Resolution: 3→3.17 Å / Redundancy: 10.9 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 3.3 / Num. unique all: 2522 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.849 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→86.71 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.003→3.081 Å / Total num. of bins used: 20
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