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Open data
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Basic information
Entry | Database: PDB / ID: 3hts | ||||||
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Title | HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX | ||||||
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![]() | TRANSCRIPTION/DNA / TRANSCRIPTION REGULATION / DNA-BINDING PROTEIN / COMPLEX (WINGED HELIX_TURN_ HELIX-DNA) / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | ![]() protein-DNA complex / cellular response to heat / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of transcription by RNA polymerase II / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Littlefield, O. / Nelson, H.C.M. | ||||||
![]() | ![]() Title: A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal. Authors: Littlefield, O. / Nelson, H.C. #1: ![]() Title: Refinedsolution Structure and Dynamics of the DNA-Binding Domain of the Heat Shock Factor from Kluyveromyces Lactis Authors: Damberger, F.F. / Pelton, J.G. / Liu, C. / Cho, H. / Harrison, C.J. / Nelson, H.C.M. / Wemmer, D.E. #2: ![]() Title: Crystal Structure of the DNA Binding Domain of the Heat Shock Transcription Factor Authors: Harrison, C.J. / Bohm, A.A. / Nelson, H.C.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 43.4 KB | Display | ![]() |
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PDB format | ![]() | 26.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451 KB | Display | ![]() |
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Full document | ![]() | 452.7 KB | Display | |
Data in XML | ![]() | 7.3 KB | Display | |
Data in CIF | ![]() | 9.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2htsS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: DNA chain | Mass: 3662.404 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: DESIGN BASED UPON HEAT SHOCK ELEMENT CONSENSUS SEQUENCE |
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#2: Protein | Mass: 11941.409 Da / Num. of mol.: 1 / Fragment: DNA BINDING DOMAIN (RESIDUES 193-281) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-GOL / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.89 Å3/Da / Density % sol: 35.02 % Description: LOW RESOLUTION DATA WERE COLLECTED ON A ROTATING ANODE | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 15, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→27.5 Å / Num. obs: 10653 / % possible obs: 89.4 % / Observed criterion σ(I): 1 / Redundancy: 3.2 % / Biso Wilson estimate: 19.9 Å2 / Rmerge(I) obs: 0.035 / Net I/σ(I): 10.3 |
Reflection shell | Resolution: 1.75→1.8 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.095 / Mean I/σ(I) obs: 7.4 / % possible all: 86 |
Reflection | *PLUS Num. measured all: 34273 |
Reflection shell | *PLUS % possible obs: 86 % / Num. unique obs: 750 / Num. measured obs: 1928 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 2HTS Resolution: 1.75→20 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.0001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
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Displacement parameters | Biso mean: 38.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.75→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.75→1.86 Å / Rfactor Rfree error: 0.025 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 10.5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 38.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.319 / % reflection Rfree: 9.5 % / Rfactor Rwork: 0.323 / Rfactor obs: 0.323 |