Type: D-saccharide, beta linking / Mass: 260.136 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Formula: C6H13O9P
Identifier
Type
Program
b-D-Glcp6PO3
IUPAC CARBOHYDRATE SYMBOL
PDB-CARE 1.0
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3 Å3/Da / Density % sol: 58.4 %
Crystal grow
Temperature: 291 K / pH: 7.2 Details: reservoir solution: 16% PEG 3350, 0.20 M Ammonium Citrate, 0.1 M HEPES. 0.005 M glucose-6-phosphate, 0.005 M ADP-Mg, 0.02 M TCEP, 1:100 (w/w) trypsin was added to the protein stock solution. ...Details: reservoir solution: 16% PEG 3350, 0.20 M Ammonium Citrate, 0.1 M HEPES. 0.005 M glucose-6-phosphate, 0.005 M ADP-Mg, 0.02 M TCEP, 1:100 (w/w) trypsin was added to the protein stock solution., pH 7.2, vapor diffusion, sitting drop, temperature 291K
Resolution: 2.8→20 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.915 / Occupancy max: 1 / Occupancy min: 1 / SU B: 48.382 / SU ML: 0.384 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THE PROGRAMS CHAINSAW, PRODRG, PHENIX, COOT AND MOLPROBLITY WERE ALSO USED.