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Yorodumi- PDB-3h4d: Ternary complex of human DNA polymerase iota with template U/T an... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3h4d | ||||||
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Title | Ternary complex of human DNA polymerase iota with template U/T and incoming dGTP | ||||||
Components |
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Keywords | REPLICATION/DNA / DNA polymerase iota / replication / DNA damage / DNA repair / DNA replication / DNA synthesis / DNA-binding / DNA-directed DNA polymerase / Magnesium / Metal-binding / Mutator protein / Nucleotidyltransferase / Nucleus / Schiff base / Transferase / REPLICATION-DNA COMPLEX | ||||||
Function / homology | Function and homology information translesion synthesis / error-prone translesion synthesis / Translesion synthesis by POLI / Termination of translesion DNA synthesis / cytoplasmic ribonucleoprotein granule / DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nuclear speck ...translesion synthesis / error-prone translesion synthesis / Translesion synthesis by POLI / Termination of translesion DNA synthesis / cytoplasmic ribonucleoprotein granule / DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nuclear speck / DNA repair / nucleoplasm / metal ion binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Jain, R. / Nair, D.T. / Johnson, R.E. / Prakash, L. / Prakash, S. / Aggarwal, A.K. | ||||||
Citation | Journal: Structure / Year: 2009 Title: Replication across template T/U by human DNA polymerase-iota. Authors: Jain, R. / Nair, D.T. / Johnson, R.E. / Prakash, L. / Prakash, S. / Aggarwal, A.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3h4d.cif.gz | 107.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3h4d.ent.gz | 76.8 KB | Display | PDB format |
PDBx/mmJSON format | 3h4d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h4/3h4d ftp://data.pdbj.org/pub/pdb/validation_reports/h4/3h4d | HTTPS FTP |
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-Related structure data
Related structure data | 3h40C 3h4bC 2alzS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 44037.047 Da / Num. of mol.: 1 / Fragment: polymerase iota Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: POLI, RAD30B / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: Q9UNA4, DNA-directed DNA polymerase |
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-DNA chain , 2 types, 2 molecules PT
#2: DNA chain | Mass: 2091.414 Da / Num. of mol.: 1 / Fragment: primer DNA strand / Source method: obtained synthetically / Details: primer DNA strand |
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#3: DNA chain | Mass: 2802.665 Da / Num. of mol.: 1 / Fragment: template DNA strand / Source method: obtained synthetically / Details: template DNA strand |
-Non-polymers , 3 types, 275 molecules
#4: Chemical | #5: Chemical | ChemComp-DGT / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.23 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 10-15% PEG 5K MME, 0.2-0.4 M Ammonium sulfate, 0.1 M MES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 0.92017 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 5, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92017 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 30091 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14.3 % / Biso Wilson estimate: 26 Å2 / Rsym value: 0.086 / Net I/σ(I): 31.4 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 14.6 % / Mean I/σ(I) obs: 5.9 / Num. unique all: 2946 / Rsym value: 0.433 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2ALZ Resolution: 2.2→36.68 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 54308.56 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 50.5225 Å2 / ksol: 0.338411 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 50.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→36.68 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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Xplor file |
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