+Open data
-Basic information
Entry | Database: PDB / ID: 3gm7 | ||||||||||||||||||||
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Title | 1.58 A resolution X-ray structure of (CUG)6 | ||||||||||||||||||||
Components | 5'-R(*Keywords | RNA / stretched U-U wobble / myotonic dystrophy / CUG repeats | Function / homology | RNA / RNA (> 10) | Function and homology information Method | X-RAY DIFFRACTION / PDB entry 1ZEV / Resolution: 1.58 Å | Authors | Kiliszek, A. / Kierzek, R. / Krzyzosiak, W.J. / Rypniewski, W. | Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2005 | Title: The structural basis of myotonic dystrophy from the crystal structure of CUG repeats. Authors: Mooers, B.H. / Logue, J.S. / Berglund, J.A. History |
Remark 0 | THIS ENTRY (3GM7) REFLECTS AN ALTERNATIVE MODELING OF THE ORIGINAL STRUCTURE (PDB ID 1ZEV) ...THIS ENTRY (3GM7) REFLECTS AN ALTERNATIVE MODELING OF THE ORIGINAL STRUCTURE (PDB ID 1ZEV) DETERMINED BY THE AUTHORS: B.H. MOOERS, J.S. LOGUE, J.A. BERGLUND | |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3gm7.cif.gz | 50.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3gm7.ent.gz | 37.8 KB | Display | PDB format |
PDBx/mmJSON format | 3gm7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gm/3gm7 ftp://data.pdbj.org/pub/pdb/validation_reports/gm/3gm7 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: RNA chain | Mass: 5694.363 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: Synthetic mRNA with the sequence of the part of human mRNA #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.83 Å3/Da / Density % sol: 32.61 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: MOPS, NaCl, MgCl2, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
-Processing
Software | Name: SHELXL-97 / Classification: refinement | ||||||||||||||||||||||||
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Refinement | Method to determine structure: PDB entry 1ZEV / Resolution: 1.58→19.6 Å / Cross valid method: THROUGHOUT Details: AUTHORS STATE THAT ALL THE ATOMS THAT ARE LISTED IN REMARK 500 FIT WELL THE ELECTRON DENSITY.
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Displacement parameters | Biso mean: 33.8 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.58→19.6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.58→1.64 Å
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