- PDB-3ghd: Crystal structure of a cystathionine beta-synthase domain protein... -
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Basic information
Entry
Database: PDB / ID: 3ghd
Title
Crystal structure of a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
Components
a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
Keywords
Nucleotide binding protein / Metal binding protein / PF1953 / APC40009 / cystathionine beta-synthase domain protein / Structural Genomics / PSI-2 / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG / METAL BINDIN
Archaeal CBS protein, (ACP)-type metal binding domain / Archaeal CBS proteins (ACP)-type metal binding (MB) domain profile. / CBS domain Like - #20 / CBS domain Like / : / Domain in cystathionine beta-synthase and other proteins. / CBS domain superfamily / CBS domain / CBS domain / CBS domain profile. ...Archaeal CBS protein, (ACP)-type metal binding domain / Archaeal CBS proteins (ACP)-type metal binding (MB) domain profile. / CBS domain Like - #20 / CBS domain Like / : / Domain in cystathionine beta-synthase and other proteins. / CBS domain superfamily / CBS domain / CBS domain / CBS domain profile. / Alpha-Beta Complex / Alpha Beta Similarity search - Domain/homology
A: a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain B: a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
A: a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain B: a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
A: a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain B: a cystathionine beta-synthase domain protein fused to a Zn-ribbon-like domain
Mass: 18.015 Da / Num. of mol.: 112 / Source method: isolated from a natural source / Formula: H2O
Sequence details
AUTHORS STATE THAT THE PROTEIN WAS CRYSTALLIZED BY IN SITU PROTEOLYSIS METHOD AND THE EXACT ...AUTHORS STATE THAT THE PROTEIN WAS CRYSTALLIZED BY IN SITU PROTEOLYSIS METHOD AND THE EXACT SEQUENCE WENT INTO THE CRYSTAL WAS NOT DETERMINED. THE PROTEIN WAS MIXED WITH 100:1(W/W) WITH CHYMOTRYPSIN IMMEDIATELY PRIOR TO CRYSTALLIZATION TRIAL. THE FULL SEQUENCE BEFORE PROTEOLYSIS WAS MGSSHHHHHHSSGRENLYFQGMAQKILVEQVVKRKAIVVQPKDTVDRVAKILSRNKAG SAVVMEGDEILGVVTERDILDKVVAKGKNPKEVKVEEIMTKNPVKIEYDYDIEDVIEL MTEKGVRRVLVTKFGKPIGFVTAADILAALASHNHEEEEEEREEESEVYGICEVCGQY GALYKVYHEGRELWVCETCKDLIEGR
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.25 Å3/Da / Density % sol: 45.44 %
Crystal grow
Temperature: 297 K / Method: vapor diffusion, hanging drop Details: 0.2M Ammonium Chloride,20%PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K
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