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- PDB-3fyr: Crystal structure of the sporulation histidine kinase inhibitor S... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3fyr | ||||||
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Title | Crystal structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis | ||||||
![]() | Sporulation inhibitor sda | ||||||
![]() | Transferase inhibitor / helical hairpin / histidine kinase inhibitor / sporulation regulation / Alternative initiation / Protein kinase inhibitor / Sporulation | ||||||
Function / homology | Sporulation inhibitor A / Sporulation inhibitor A superfamily / Sporulation inhibitor A / sporulation resulting in formation of a cellular spore / protein kinase inhibitor activity / Sporulation inhibitor sda![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jacques, D.A. / Streamer, M. / King, G.F. / Guss, J.M. / Trewhella, J. / Langley, D.B. | ||||||
![]() | ![]() Title: Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state Authors: Jacques, D.A. / Streamer, M. / Rowland, S.L. / King, G.F. / Guss, J.M. / Trewhella, J. / Langley, D.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 31.4 KB | Display | ![]() |
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PDB format | ![]() | 24.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.8 KB | Display | ![]() |
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Full document | ![]() | 430.8 KB | Display | |
Data in XML | ![]() | 6 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | THE DEPOSITORS HAVE IN DEPENDANT DATA WHICH SUGGESTS THAT IN SOLUTION THE OLIGOMERIC STATE OF THE ASU (IE AN ODD-LOOKING TRIMER) BEST FITS SAXS DATA OF THE PROTEIN IN SOLUTION. |
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Components
#1: Protein/peptide | Mass: 5686.198 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Sequence details | THE FIRST 6 RESIDUES, MNWVPS, ARE MISSING IN NATURAL ACCORDING TO REFERENCE 2, SDA_BACSU IN UNIPROT. ...THE FIRST 6 RESIDUES, MNWVPS, ARE MISSING IN NATURAL ACCORDING TO REFERENCE 2, SDA_BACSU IN UNIPROT. THERE IS AN ALTERNATE START CODON WHICH THE DEPOSITORS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.718006 Å3/Da / Density % sol: 28.405363 % / Mosaicity: 0.639 ° |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.3 Details: equal volumes of protein solution (7.5 mg/ml) and well solution (0.1M MES, pH 6.3, 15% (w/v) PEG 5000MME) were combined., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Feb 20, 2008 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength |
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Reflection | Redundancy: 3.6 % / Av σ(I) over netI: 14.87 / Number: 56720 / Rmerge(I) obs: 0.06 / Χ2: 1 / D res high: 1.97 Å / D res low: 50 Å / Num. obs: 15638 / % possible obs: 99.5 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Diffraction reflection shell |
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Reflection | Resolution: 1.97→50 Å / Num. obs: 8936 / % possible obs: 99.5 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.06 / Χ2: 1.005 / Net I/σ(I): 14.873 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Resolution: 1.97→2.04 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.564 / Mean I/σ(I) obs: 2 / Num. unique all: 1577 / Χ2: 1.029 / % possible all: 99.9 |
-Phasing
Phasing | Method: ![]() | ||||||||||||||||||||||||||||
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Phasing set |
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Phasing MAD set |
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Phasing MAD set site |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS; U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 77.98 Å2 / Biso mean: 37.902 Å2 / Biso min: 21.37 Å2
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Refinement step | Cycle: LAST / Resolution: 1.97→27.69 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.971→2.022 Å / Total num. of bins used: 20
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