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- PDB-3flc: Crystal structure of the His-tagged H232R mutant of glycerol kina... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3flc | ||||||
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Title | Crystal structure of the His-tagged H232R mutant of glycerol kinase from Enterococcus casseliflavus with glycerol | ||||||
![]() | Glycerol kinase | ||||||
![]() | TRANSFERASE / ATP-binding / Glycerol metabolism / Kinase / Nucleotide-binding / Phosphoprotein | ||||||
Function / homology | ![]() glycerol kinase / glycerol-3-phosphate biosynthetic process / glycerol kinase activity / glycerol metabolic process / glycerol catabolic process / triglyceride metabolic process / phosphorylation / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Briozzo, P. | ||||||
![]() | ![]() Title: Structural Characterizations of Glycerol Kinase: Unraveling Phosphorylation-Induced Long-Range Activation Authors: Yeh, J.I. / Kettering, R. / Saxl, R. / Bourand, A. / Darbon, E. / Joly, N. / Briozzo, P. / Deutscher, J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 215.6 KB | Display | ![]() |
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PDB format | ![]() | 171.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464.5 KB | Display | ![]() |
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Full document | ![]() | 486.9 KB | Display | |
Data in XML | ![]() | 44.7 KB | Display | |
Data in CIF | ![]() | 65.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3d7eC ![]() 3h3nC ![]() 3h3oC ![]() 3h45C ![]() 3h46C ![]() 1r59S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 57237.070 Da / Num. of mol.: 2 / Mutation: H232R Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.25 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 2.0M ammonium sulfate, 5%(v/v) isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD / Date: Mar 17, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.931 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→30 Å / Num. obs: 87047 / % possible obs: 97.8 % / Observed criterion σ(I): 2 / Redundancy: 18.25 % / Rsym value: 0.073 / Net I/σ(I): 16.2 |
Reflection shell | Resolution: 1.85→1.92 Å / Mean I/σ(I) obs: 2.15 / Rsym value: 0.598 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1R59, molecule O Resolution: 1.85→30 Å / σ(F): 2 / Stereochemistry target values: CNS
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Refinement step | Cycle: LAST / Resolution: 1.85→30 Å
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Refine LS restraints |
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