- PDB-3ffs: The Crystal Structure of Cryptosporidium parvum Inosine-5'-Monoph... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 3ffs
Title
The Crystal Structure of Cryptosporidium parvum Inosine-5'-Monophosphate Dehydrogenase
Components
Inosine-5-monophosphate dehydrogenase
Keywords
OXIDOREDUCTASE / beta-alpha barrel / TIM fold
Function / homology
Function and homology information
IMP dehydrogenase activity / IMP dehydrogenase / GMP biosynthetic process / GTP biosynthetic process / metal ion binding / cytoplasm Similarity search - Function
Component-ID: 1 / Beg auth comp-ID: THR / Beg label comp-ID: THR / End auth comp-ID: THR / End label comp-ID: THR / Refine code: 6 / Auth seq-ID: 3 - 378 / Label seq-ID: 3 - 378
Dom-ID
Ens-ID
Auth asym-ID
Label asym-ID
1
1
A
A
2
1
B
B
3
1
C
C
4
1
D
D
1
2
B
B
2
2
A
A
3
2
C
C
4
2
D
D
1
3
C
C
2
3
A
A
3
3
B
B
4
3
D
D
1
4
D
D
2
4
A
A
3
4
B
B
4
4
C
C
NCS ensembles :
ID
1
2
3
4
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Components
#1: Protein
Inosine-5-monophosphatedehydrogenase
Mass: 43134.262 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Cryptosporidium parvum (eukaryote) / Gene: IMPDH, 56k.02 / Plasmid: pTacTac / Production host: Escherichia coli (E. coli) / Strain (production host): TX685 / References: UniProt: Q8T6T2, IMP dehydrogenase
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.6 Å3/Da / Density % sol: 52.66 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: Protein solution: 4 mg/mL IMPDH, 50 mM Tris, pH 7.5, 150 mM KCl, 5 % glycerol and 2 mM dithiothreitol. Well solution: 34 % PEG 4000, 25 mM sodium acetate and 100 mM Tris-Cl, pH 8.5. Combined ...Details: Protein solution: 4 mg/mL IMPDH, 50 mM Tris, pH 7.5, 150 mM KCl, 5 % glycerol and 2 mM dithiothreitol. Well solution: 34 % PEG 4000, 25 mM sodium acetate and 100 mM Tris-Cl, pH 8.5. Combined in a 1:1 ratio. VAPOR DIFFUSION, HANGING DROP, temperature 298K