+Open data
-Basic information
Entry | Database: PDB / ID: 3f7d | ||||||
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Title | SF-1 LBD bound by phosphatidylcholine | ||||||
Components |
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Keywords | TRANSCRIPTION / nuclear receptor / coactivator peptide / ligand / phospholipid / phosphatidylcholine / transcriptional regulation / DNA-binding / Metal-binding / Nucleus / Receptor / Transcription regulation / Zinc-finger / Activator / RNA-binding | ||||||
Function / homology | Function and homology information flavone metabolic process / : / : / positive regulation of cellular metabolic process / response to metformin / response to norepinephrine / : / euchromatin => GO:0000791 / cellular response to resveratrol / cellular response to nitrite ...flavone metabolic process / : / : / positive regulation of cellular metabolic process / response to metformin / response to norepinephrine / : / euchromatin => GO:0000791 / cellular response to resveratrol / cellular response to nitrite / response to gonadotropin-releasing hormone / positive regulation of progesterone biosynthetic process / Sertoli cell differentiation / response to electrical stimulus involved in regulation of muscle adaptation / reproductive process / : / cellular response to ionomycin / SUMOylation of intracellular receptors / negative regulation of female gonad development / Nuclear Receptor transcription pathway / positive regulation of mitochondrial DNA metabolic process / positive regulation of muscle tissue development / skeletal muscle atrophy / sex determination / response to methionine / positive regulation of male gonad development / positive regulation of podocyte apoptotic process / negative regulation of smooth muscle cell migration / luteinization / adaptive thermogenesis / calcineurin-mediated signaling / : / positive regulation of cellular respiration / response to xenobiotic stimulus => GO:0009410 / response to epinephrine / positive regulation of fatty acid oxidation / tissue development / chromatin => GO:0000785 / cellular response to fructose stimulus / cellular response to follicle-stimulating hormone stimulus / response to L-leucine / cellular response to potassium ion / autophagy of mitochondrion / Leydig cell differentiation / galactose metabolic process / : / male sex determination / apical dendrite / maintenance of protein location in nucleus / cellular response to interleukin-6 / hormone metabolic process / cellular response to thyroid hormone stimulus / negative regulation of glycolytic process / peroxisome proliferator activated receptor binding / response to muscle activity / adrenal gland development / female gonad development / positive regulation of ATP biosynthetic process / response to starvation / cellular response to caffeine / fatty acid oxidation / regulation of NMDA receptor activity / response to dietary excess / transcription factor binding / alpha-tubulin binding / androgen metabolic process / negative regulation of mitochondrial fission / adipose tissue development / energy homeostasis / brown fat cell differentiation / forebrain development / negative regulation of signaling receptor activity / cellular response to transforming growth factor beta stimulus / respiratory electron transport chain / response to cold / hormone-mediated signaling pathway / cerebellum development / mitochondrion organization / cytosolic ribosome / response to reactive oxygen species / nuclear receptor coactivator activity / negative regulation of protein phosphorylation / response to activity / cellular response to estradiol stimulus / response to ischemia / gluconeogenesis / promoter-specific chromatin binding / transcription coregulator binding / nuclear estrogen receptor binding / nuclear receptor binding / cellular response to glucose stimulus / transcription coregulator activity / negative regulation of smooth muscle cell proliferation / RNA polymerase II transcription regulatory region sequence-specific DNA binding / positive regulation of smooth muscle cell proliferation / phospholipid binding / circadian regulation of gene expression / regulation of circadian rhythm / PML body / chromatin DNA binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Sablin, E.P. / Fletterick, R.J. | ||||||
Citation | Journal: Mol.Endocrinol. / Year: 2009 Title: Structure of SF-1 bound by different phospholipids: evidence for regulatory ligands. Authors: Sablin, E.P. / Blind, R.D. / Krylova, I.N. / Ingraham, J.G. / Cai, F. / Williams, J.D. / Fletterick, R.J. / Ingraham, H.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3f7d.cif.gz | 120.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3f7d.ent.gz | 92.7 KB | Display | PDB format |
PDBx/mmJSON format | 3f7d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f7/3f7d ftp://data.pdbj.org/pub/pdb/validation_reports/f7/3f7d | HTTPS FTP |
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-Related structure data
Related structure data | 1ymtS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 27968.135 Da / Num. of mol.: 1 / Fragment: UNP residues 219-462 / Mutation: C302S, C408S, C413S, C423S, C267(CAF) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Nr5a1, Ftzf1, RP23-354G20.5-001 / Production host: Escherichia coli (E. coli) / References: UniProt: Q812G5, UniProt: P33242*PLUS |
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#2: Protein/peptide | Mass: 1523.854 Da / Num. of mol.: 1 / Fragment: UNP residues 137-150 / Source method: obtained synthetically / Source: (synth.) Mus musculus (house mouse) / References: UniProt: O70343 |
#3: Chemical | ChemComp-P42 / ( |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.34 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1 M sodium cacodylate, 20% PEG 3350, 0.2 M sodium acetate, 2 mM TCEP, 10% xylitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 25, 2005 / Details: mirrors |
Radiation | Monochromator: KHOZU Double flat crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→25 Å / Num. all: 13803 / Num. obs: 13232 / % possible obs: 95.86 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.7 % / Biso Wilson estimate: 26.5 Å2 / Rmerge(I) obs: 0.07 / Rsym value: 0.07 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.24 / Mean I/σ(I) obs: 3.3 / Rsym value: 0.24 / % possible all: 89.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDb entry 1YMT Resolution: 2.2→23.76 Å / SU ML: 0.32 / σ(F): 0.06 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 47.77 Å2 / ksol: 0.34 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→23.76 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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