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- PDB-3f3g: Crystal structure of the nucleoporin pair Nup85-Seh1, space group... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3f3g | ||||||
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Title | Crystal structure of the nucleoporin pair Nup85-Seh1, space group P212121 | ||||||
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![]() | STRUCTURAL PROTEIN / Protein Complex / Nucleoporin / Nucleoporin Complex / Nuclear Pore Complex / Macromolecular Assembly / Membrane Coat / Nucleocytoplasmic Transport / beta-propeller / solenoid domain / mRNA transport / Nucleus / Protein transport / Translocation / WD repeat | ||||||
Function / homology | ![]() Seh1-associated complex / nuclear pore localization / regulation of TORC1 signaling / nuclear pore outer ring / Transport of Mature mRNA derived from an Intron-Containing Transcript / Regulation of HSF1-mediated heat shock response / SUMOylation of SUMOylation proteins / SUMOylation of RNA binding proteins / structural constituent of nuclear pore / SUMOylation of chromatin organization proteins ...Seh1-associated complex / nuclear pore localization / regulation of TORC1 signaling / nuclear pore outer ring / Transport of Mature mRNA derived from an Intron-Containing Transcript / Regulation of HSF1-mediated heat shock response / SUMOylation of SUMOylation proteins / SUMOylation of RNA binding proteins / structural constituent of nuclear pore / SUMOylation of chromatin organization proteins / nucleocytoplasmic transport / vacuolar membrane / ribosomal large subunit export from nucleus / mRNA transport / mRNA export from nucleus / nuclear pore / positive regulation of TORC1 signaling / cellular response to amino acid starvation / protein import into nucleus / nuclear envelope / protein transport / nuclear membrane / positive regulation of DNA-templated transcription Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Debler, E.W. / Hseo, H. / Ma, Y. / Blobel, G. / Hoelz, A. | ||||||
![]() | ![]() Title: A fence-like coat for the nuclear pore membrane. Authors: Debler, E.W. / Ma, Y. / Seo, H.S. / Hsia, K.C. / Noriega, T.R. / Blobel, G. / Hoelz, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 612.9 KB | Display | ![]() |
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PDB format | ![]() | 501.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3f3fSC ![]() 3f3pC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The biological unit is the complete heterooctamer that is present in the asymmetric unit |
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Components
#1: Protein | Mass: 39406.016 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: SEH1 / Plasmid: pETDuet-1 / Production host: ![]() ![]() #2: Protein | Mass: 64929.242 Da / Num. of mol.: 4 / Fragment: UNP residues 1-570 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: NUP85, RAT9 / Plasmid: pETDuet-1 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.98 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop Details: Sodium citrate, Sodium chloride, Tris-HCl buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 5, 2007 |
Radiation | Monochromator: double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03319 Å / Relative weight: 1 |
Reflection | Resolution: 3.75→50 Å / Num. all: 48007 / Num. obs: 46183 / % possible obs: 99.2 % / Observed criterion σ(I): -3 / Redundancy: 5.4 % / Rsym value: 0.18 / Net I/σ(I): 7.8 |
Reflection shell | Resolution: 3.75→3.88 Å / Redundancy: 4.6 % / Mean I/σ(I) obs: 1.9 / Rsym value: 0.753 / % possible all: 94.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3F3F Resolution: 3.75→50 Å / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.75→50 Å
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Refine LS restraints |
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