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- PDB-3ewa: RADA recombinase from METHANOCOCCUS MARIPALUDIS in complex with A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ewa | ||||||
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Title | RADA recombinase from METHANOCOCCUS MARIPALUDIS in complex with AMPPNP and ammonium ions | ||||||
![]() | DNA repair and recombination protein radA | ||||||
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Function / homology | ![]() DNA recombinase assembly / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, Y. / He, Y. / Luo, Y. | ||||||
![]() | ![]() Title: Conservation of a conformational switch in RadA recombinase from Methanococcus maripaludis. Authors: Li, Y. / He, Y. / Luo, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 75.6 KB | Display | ![]() |
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PDB format | ![]() | 55.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3etlC ![]() 3ew9C ![]() 1t4gS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | authors state that infinite helical assembly can be generated by applying the crystallographic 6-fold symmetry |
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Components
#1: Protein | Mass: 35205.168 Da / Num. of mol.: 1 / Mutation: I124M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-ANP / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.45 % |
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Crystal grow![]() | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7.9 Details: 8% PEg 3350, 0.05 M MgCl2, 0.4 M NH4Cl, 0.05 M Tris-HCl, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Aug 22, 2007 / Details: BENDING CRYSTAL |
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→20 Å / Num. all: 27651 / Num. obs: 26987 / % possible obs: 97.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.7 % / Rsym value: 0.061 / Net I/σ(I): 17.5 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 10.6 % / Num. unique all: 3375 / Rsym value: 0.448 / % possible all: 91.6 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1T4G Resolution: 2→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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