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- PDB-2fpm: RadA recombinase in complex with AMP-PNP and high concentration of K+ -
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Open data
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Basic information
Entry | Database: PDB / ID: 2fpm | |||||||||
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Title | RadA recombinase in complex with AMP-PNP and high concentration of K+ | |||||||||
![]() | DNA repair and recombination protein radA | |||||||||
![]() | RECOMBINATION / ATPASE / PROTEIN-ATP COMPLEX / RADA-ADP COMPLEX / CO-FACTORS / POTASSIUM-DEPENDENCE | |||||||||
Function / homology | ![]() DNA recombinase assembly / mitotic recombination / DNA strand invasion / DNA strand exchange activity / ATP-dependent DNA damage sensor activity / single-stranded DNA binding / double-stranded DNA binding / damaged DNA binding / ATP hydrolysis activity / ATP binding / identical protein binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Wu, Y. / Qian, X. / He, Y. / Moya, I.A. / Luo, Y. | |||||||||
![]() | ![]() Title: Crystal Structure of Methanoccocus Voltae Rada in Complex with Adp: hydrolysis-induced conformational change Authors: Qian, X. / Wu, Y. / He, Y. / Moya, I.A. / Luo, Y. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79 KB | Display | ![]() |
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PDB format | ![]() | 57 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 748.5 KB | Display | ![]() |
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Full document | ![]() | 756.5 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 21.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2fpkC ![]() 2fplC ![]() 1pznS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35198.930 Da / Num. of mol.: 1 / Mutation: S2G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-ANP / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.4 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 7% PEG 3350, 50 MM HEPES BUFFER, 50 MM MGCL2, 0.6 M K AC, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: ENRAF-NONIUS / Detector: CCD / Date: Jan 16, 2004 / Details: MIRRORS |
Radiation | Monochromator: BRUKERS MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→40 Å / Num. all: 28274 / Num. obs: 28217 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.5 % / Rmerge(I) obs: 0.039 / Rsym value: 0.039 / Net I/σ(I): 14.9 |
Reflection shell | Resolution: 2→2.1 Å / Rmerge(I) obs: 0.279 / Rsym value: 0.279 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1PZN Resolution: 2→20 Å / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: Engh & Huber / Details: CNS REFINEMENT
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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Xplor file |
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