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- PDB-3enu: Crystal structure of Nitrollin, a betagamma-crystallin from Nitro... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3enu | ||||||
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Title | Crystal structure of Nitrollin, a betagamma-crystallin from Nitrosospira multiformis | ||||||
![]() | Putative uncharacterized protein | ||||||
![]() | STRUCTURAL PROTEIN / betagamma crystallin | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Aravind, P. / Sankaranarayanan, R. | ||||||
![]() | ![]() Title: Three-dimensional domain swapping in nitrollin, a single-domain betagamma-crystallin from Nitrosospira multiformis, controls protein conformation and stability but not dimerization Authors: Aravind, P. / Suman, S.K. / Mishra, A. / Sharma, Y. / Sankaranarayanan, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 34.6 KB | Display | ![]() |
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PDB format | ![]() | 25.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.8 KB | Display | ![]() |
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Full document | ![]() | 420.3 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 10.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12949.712 Da / Num. of mol.: 1 Fragment: single domain betagamma-crystallin, residues 27-140 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.02 % / Mosaicity: 0.486 ° |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 20% PEG3350, 0.2M Naformate, vapor diffusion, sitting drop, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MAR 345dtb / Detector: IMAGE PLATE / Date: Oct 4, 2007 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.86→25 Å / Num. obs: 10654 / % possible obs: 99.8 % / Redundancy: 6.6 % / Rmerge(I) obs: 0.046 / Χ2: 1.109 / Net I/σ(I): 40.15 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Phasing
Phasing | Method: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||
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Phasing MIR | Resolution: 2.4→20 Å / FOM: 0.51 / Reflection: 5062 | ||||||||||||||||||||||||||||||||||||||||||||||||
Phasing MIR der |
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Phasing MIR der site |
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Phasing MIR shell |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Bsol: 42.54 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso max: 44.65 Å2 / Biso mean: 11.501 Å2 / Biso min: 3 Å2
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Refinement step | Cycle: LAST / Resolution: 1.86→25 Å
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Refine LS restraints |
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Xplor file |
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