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Open data
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Basic information
Entry | Database: PDB / ID: 3ebk | ||||||
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Title | Crystal structure of major allergens, Bla g 4 from cockroaches | ||||||
![]() | Allergen Bla g 4 | ||||||
![]() | ALLERGEN / Beta barrel / Cockroach / Glycoprotein / Secreted | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tan, Y.W. / Chan, S.L. / Chew, F.T. / Sivaraman, J. / Mok, Y.K. | ||||||
![]() | ![]() Title: Structures of two major allergens, Bla g 4 and Per a 4, from cockroaches and their IgE binding epitopes. Authors: Tan, Y.W. / Chan, S.L. / Ong, T.C. / Yit, L.Y. / Tiong, Y.S. / Chew, F.T. / Sivaraman, J. / Mok, Y.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 78.6 KB | Display | ![]() |
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PDB format | ![]() | 63.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 432.5 KB | Display | ![]() |
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Full document | ![]() | 439.7 KB | Display | |
Data in XML | ![]() | 16.4 KB | Display | |
Data in CIF | ![]() | 22.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20375.754 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.67 % / Mosaicity: 0.396 ° |
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Crystal grow | Temperature: 298 K / Method: hanging drop / pH: 7.5 Details: 0.1M HEPES, 1.5M sodium citrate, 3% acetone, pH 7.5, hanging drop, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC / Detector: CCD / Date: Jan 1, 2006 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.75→50 Å / Num. obs: 45386 / % possible obs: 99.9 % / Redundancy: 15 % / Rmerge(I) obs: 0.064 / Χ2: 1.082 / Net I/σ(I): 43.189 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Bsol: 47.226 Å2 | ||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 105.32 Å2 / Biso mean: 35.961 Å2 / Biso min: 16.4 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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Xplor file |
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