PDB-3e2l: Crystal Structure of the KPC-2 Beta-lactamase/Beta-lactamase inhi...

ID or keywords:

Entry

Database: PDB / ID: 3e2l

Title

Crystal Structure of the KPC-2 Beta-lactamase/Beta-lactamase inhibitor protein (BLIP)

Components

Beta-lactamase inhibitory protein
Carbapenemase

Keywords

HYDROLASE/HYDROLASE INHIBITOR / Beta-lactamase / Beta-lactamase inhibitor protein / protein-protein complex / BLIP / KPC-2 / Antibiotic resistance / Hydrolase / Plasmid / Secreted / HYDROLASE-HYDROLASE INHIBITOR COMPLEX

Function / homology

Function and homology information

regulation of beta-lactamase activity / beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / response to antibiotic / extracellular region
Similarity search - Function

Biological species

Klebsiella pneumoniae (bacteria)

Streptomyces clavuligerus (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 1.87 Å

Authors

Hanes, M.S. / Jude, K.M. / Berger, J.M. / Kirsch, J.F. / Bonomo, R.A. / Handel, T.M.

Citation

Journal: Biochemistry / Year: 2009
Title: Structural and biochemical characterization of the interaction between KPC-2 beta-lactamase and beta-lactamase inhibitor protein
Authors: Hanes, M.S. / Jude, K.M. / Berger, J.M. / Bonomo, R.A. / Handel, T.M.

History

Deposition	Aug 5, 2008	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 4, 2009	Provider: repository / Type: Initial release
Revision 1.1	Jul 13, 2011	Group: Version format compliance
Revision 1.2	Oct 25, 2017	Group: Refinement description / Category: software
Revision 1.3	Oct 20, 2021	Group: Database references / Derived calculations / Category: database_2 / struct_conn / struct_ref_seq_dif Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ref_seq_dif.details

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	3e2l.cif.gz	335.4 KB	Display	PDBx/mmCIF format
PDB format	pdb3e2l.ent.gz	278.3 KB	Display	PDB format
PDBx/mmJSON format	3e2l.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	3e2l_validation.pdf.gz	445.6 KB	Display	wwPDB validaton report
Full document	3e2l_full_validation.pdf.gz	450.7 KB	Display
Data in XML	3e2l_validation.xml.gz	37.5 KB	Display
Data in CIF	3e2l_validation.cif.gz	56 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/e2/3e2l ftp://data.pdbj.org/pub/pdb/validation_reports/e2/3e2l	HTTPS FTP

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Related structure data

Related structure data	3e2kC 1jtg 2g2u 2ov5 C: citing same article (ref.)
Similar structure data	3e2k-1 1w9h-d 5cwu-1 3h4t-d 6kd5-d 5cwu-8 3zlf-2 2bvf-1 6cwf-d 1xxm-2 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#187 - Jul 2015 New Delhi Metallo-β-Lactamase similarity (3) #164 - Aug 2013 Serotonin Receptor similarity (3) #29 - May 2002 Penicillin-binding Proteins similarity (1)

Deposited unit

A: Carbapenemase

B: Carbapenemase

C: Beta-lactamase inhibitory protein

D: Beta-lactamase inhibitory protein

91.2 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Carbapenemase

C: Beta-lactamase inhibitory protein

defined by author&software
45.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Carbapenemase

D: Beta-lactamase inhibitory protein

defined by author&software
45.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	162.243, 66.391, 82.205
Angle α, β, γ (deg.)	90.00, 101.28, 90.00
Int Tables number	5
Space group name H-M	C121

#1: Protein	Carbapenemase / Class A carbapenemase / Beta-lactamase / Class A carbapenemase KPC-2 Mass: 28054.275 Da / Num. of mol.: 2 / Fragment: UNP residues 30-293 / Mutation: G175S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Klebsiella pneumoniae (bacteria) / Gene: KPC-2, blaKPC-2 / Plasmid: pET24a(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: Q93LQ9, UniProt: Q9F663*PLUS, beta-lactamase
#2: Protein	Beta-lactamase inhibitory protein / BLIP Mass: 17556.492 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces clavuligerus (bacteria) / Plasmid: pET26b(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P35804
#3: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 702 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.38 Å³/Da / Density % sol: 48.38 %
Crystal grow	Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 20% PEG 8000, 6% ethylene glycol, 100mM citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1159 Å
Detector	Type: ADSC QUANTUM 210 / Detector: CCD / Date: Apr 6, 2008
Radiation	Monochromator: DOUBLE CRYSTAL SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1.1159 Å / Relative weight: 1
Reflection	Resolution: 1.87→200 Å / Num. all: 71225 / Num. obs: 70092 / % possible obs: 98.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.3 % / R_merge(I) obs: 0.084
Reflection shell	Resolution: 1.87→1.94 Å / Redundancy: 2.6 % / R_merge(I) obs: 0.463 / % possible all: 90.3

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Phasing

Phasing

Method:

molecular replacement

Phasing MR

Model details: Phaser MODE: MR_AUTO

	Highest resolution	Lowest resolution
Rotation	2.5 Å	51.79 Å
Translation	2.5 Å	51.79 Å

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Processing

Software

Name	Version	Classification
DENZO		data reduction
SCALEPACK		data scaling
PHASER		phasing
PHENIX		refinement
PDB_EXTRACT	3.006	data extraction
Blu-Ice		data collection
HKL-2000		data reduction
HKL-2000		data scaling

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 1.87→51.786 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 1.61 / σ(F): 0.06 / Phase error: 21.17 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2128	3376	5.07 %
R_work	0.1688	-	-
obs	0.1711	66546	93.27 %
all	-	71225	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / B_sol: 49.929 Å² / k_sol: 0.337 e/Å³

Displacement parameters

B_iso mean: 26.226 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	1.88 Å²	-0 Å²	4.373 Å²
2-	-	-1.514 Å²	0 Å²
3-	-	-	-0.366 Å²

Refinement step

Cycle: LAST / Resolution: 1.87→51.786 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6307	0	0	702	7009

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.008	6451
X-RAY DIFFRACTION	f_angle_d	1.124	8775
X-RAY DIFFRACTION	f_dihedral_angle_d	17.175	2236
X-RAY DIFFRACTION	f_chiral_restr	0.074	985
X-RAY DIFFRACTION	f_plane_restr	0.005	1139

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.87-1.8935	0.3264	109	0.2531	1916	X-RAY DIFFRACTION	69
1.8935-1.9218	0.274	101	0.252	2185	X-RAY DIFFRACTION	78
1.9218-1.9518	0.3107	124	0.2328	2311	X-RAY DIFFRACTION	81
1.9518-1.9838	0.2837	131	0.2102	2398	X-RAY DIFFRACTION	87
1.9838-2.018	0.2553	152	0.1948	2538	X-RAY DIFFRACTION	91
2.018-2.0547	0.2077	151	0.1917	2575	X-RAY DIFFRACTION	92
2.0547-2.0942	0.2338	149	0.1826	2547	X-RAY DIFFRACTION	92
2.0942-2.137	0.2225	142	0.1724	2673	X-RAY DIFFRACTION	94
2.137-2.1834	0.2319	127	0.1669	2642	X-RAY DIFFRACTION	95
2.1834-2.2342	0.2267	124	0.1648	2680	X-RAY DIFFRACTION	95
2.2342-2.2901	0.2236	154	0.1736	2611	X-RAY DIFFRACTION	93
2.2901-2.352	0.2274	114	0.1725	2695	X-RAY DIFFRACTION	95
2.352-2.4212	0.229	147	0.1657	2689	X-RAY DIFFRACTION	96
2.4212-2.4994	0.2301	181	0.167	2651	X-RAY DIFFRACTION	96
2.4994-2.5887	0.2212	127	0.1654	2737	X-RAY DIFFRACTION	97
2.5887-2.6923	0.2127	138	0.1636	2749	X-RAY DIFFRACTION	97
2.6923-2.8149	0.2249	142	0.1721	2792	X-RAY DIFFRACTION	98
2.8149-2.9633	0.2303	146	0.1756	2757	X-RAY DIFFRACTION	98
2.9633-3.1489	0.1938	161	0.1739	2796	X-RAY DIFFRACTION	98
3.1489-3.392	0.2348	137	0.1687	2820	X-RAY DIFFRACTION	99
3.392-3.7332	0.1931	136	0.1536	2835	X-RAY DIFFRACTION	99
3.7332-4.2732	0.1737	172	0.1338	2799	X-RAY DIFFRACTION	100
4.2732-5.3829	0.147	162	0.1265	2847	X-RAY DIFFRACTION	100
5.3829-51.8059	0.1799	149	0.1527	2927	X-RAY DIFFRACTION	99

Refinement TLS params.

Refine-ID: X-RAY DIFFRACTION

ID	Method	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	refined	0.1805	-0.0301	-0.006	0.1003	-0.0194	0.1299	-0.0491	-0.5607	0.0875	0.1608	0.0414	0.0362	-0.0485	0.1445	0.0451	0.1802	-0.0496	-0.0413	0.2648	-0.0531	0.0841	41.1329	-10.7537	-20.0553
2		0.1324	0.0366	0.0656	0.0525	0.0819	0.0301	0.1707	-0.172	0.1269	-0.221	0.1141	0.1629	0.074	0.0862	-0.0002	0.1728	-0.0115	0.02	0.1374	-0.0013	0.1182
3		0.461	0.0225	0.1548	-0.0655	-0.0003	0.3766	0.042	0.046	0.0207	0.0106	-0.0395	-0.0098	-0.0006	0.1019	0.0839	0.083	-0.0133	-0.0056	0.0948	-0.0022	0.0594
4		0.2799	-0.0047	0.1088	-0.0071	-0.1271	0.0315	-0.1989	0.1765	-0.0166	-0.4706	0.0959	-0.1333	0.4249	-0.2623	0.0001	0.2292	-0.0605	-0.0116	0.1901	-0.0245	0.1188
5		0.1755	-0.1116	0.2163	0.2678	-0.2156	0.0811	-0.0063	-0.0241	-0.0008	-0.0167	0.0544	0.0316	0.0833	-0.1151	0.0021	0.1221	-0.0302	-0.0006	0.1122	0.0001	0.0785
6		0.0168	-0.0936	-0.0254	0.0623	-0.0296	0.0024	0.1814	0.3479	-0.2587	-0.0766	-0.133	0.1978	0.0208	0.1127	0.0039	0.1015	0.0099	-0.0145	0.1045	-0.0373	0.0904
7		0.0065	-0.1403	0	-0.002	0.1201	0.348	-0.0066	0.0973	-0.0104	0.0731	0.0825	0.0204	0.017	0.071	0	0.105	-0.0008	-0.0085	0.1537	-0.0094	0.0904
8		0.3194	0.0505	0.0275	0.0826	-0.1462	0.7422	0.0929	-0.0157	0.0238	0.0646	-0.037	0.0592	-0.1027	0.0723	0.2146	0.1066	-0.0062	0.0129	0.0894	-0.0019	0.07
9		0.5342	0.2344	-0.2093	-0.028	-0.2012	0.2919	0.1024	0.0198	0.0447	0.0315	-0.0642	0.0527	-0.0625	0.0683	0.0852	0.1133	-0.001	0.003	0.1086	-0.002	0.0629
10		0.3789	0.4473	-0.0825	0.3977	-0.0293	0.5929	0.1378	-0.126	-0.0352	0.1506	0.0134	0.3025	-0.2043	0.1113	0.2624	0.1467	-0.0126	0.0401	0.0996	-0.0035	0.0838
11		0.0446	0.0066	0.0047	0.0311	-0	0.0213	-0.098	-0.1936	-0.1737	0.1969	0.2052	0.0494	0.2769	-0.1155	0.0001	0.1869	0.009	-0.0259	0.1892	0.0414	0.1446
12		0.0884	-0.0445	-0.0149	-0.0618	-0.0525	0.3819	0.1204	-0.0457	0.0762	0.0521	0.0388	0.0094	-0.1102	0.1092	0.3851	0.179	-0.0202	0.024	0.147	-0.017	0.0546
13		0.0204	-0.0004	-0.0017	0.0137	0.0227	0.0052	0.0531	-0.0136	0.008	0.4086	-0.1222	0.1017	-0.2545	0.0145	-0	0.4104	-0.0451	0.0904	0.1503	-0.0389	0.1566
14		0.0024	-0.0157	-0.0138	0.0093	-0.0319	-0.0032	-0.3335	-0.2319	-0.6078	0.7514	-0.0305	0.4209	0.1169	0.0337	-0	0.6158	0.0019	0.3863	0.2727	0.0667	0.4961
15		0.0073	-0.0397	-0.0004	0.0185	-0.0463	0.0282	0.1531	0.1031	-0.1561	-0.1977	0.5992	0.2835	0.1149	-0.2042	0.0004	0.2784	-0.1002	0.049	0.2627	-0.0276	0.7288
16		0.0659	-0.1112	0.1331	0.1444	-0.0942	0.4139	-0.1671	0.3129	0.0755	0.0864	0.2699	0.8576	0.0678	-0.3059	-0.0201	0.1444	-0.073	0.1215	0.2757	-0.0079	0.6279
17		0.6449	-0.49	0.1383	0.452	0.0078	0.3605	-0.3252	-0.1017	-0.1302	-0.5331	0.5289	1.0216	-0.1405	-0.1341	0.0914	0.3159	-0.2138	-0.2521	0.1592	-0.0187	0.2488
18		0.8933	-0.1163	0.1747	0.132	0.0156	0.1204	-0.2086	0.4007	0.4601	-0.773	0.0509	-0.2437	-0.0288	-0.1589	-0.0091	0.5448	-0.1507	-0.0203	0.3074	0.0834	0.088
19		0.2602	0.0108	-0.0127	0.2772	-0.0398	0.0703	-0.2145	0.0336	-0.0181	-0.1925	0.0775	-0.0515	0.1261	0.1168	0.0001	0.1865	-0.0399	-0.0175	0.2229	0.0256	0.211
20		0.0197	0.0163	0.014	-0.0074	-0.0499	-0.003	-0.0637	0.1294	0.0723	-0.8982	0.234	0.3258	0.1561	-0.1377	0.0106	0.2609	-0.0727	-0.2693	0.393	0.1057	0.3241
21		-0.0064	0.0287	-0.0005	0.0046	-0.0029	-0.024	-0.045	0.194	0.0698	-0.1847	0.5223	0.7657	0.4189	-0.2174	0.0362	0.2579	-0.1577	-0.3194	0.4525	0.0382	0.7839
22		0.0159	0.1206	0.0236	0.1046	0.0824	0.0237	-0.0037	0.2441	0.5518	0.1028	0.3017	0.996	0.1266	-0.0338	0	0.1498	0.0152	0.0276	0.2239	0.0809	0.5783
23		0.066	-0.0034	0.002	0.0171	0.0314	0.0391	0.0241	0.1196	0.2976	0.0545	0.2243	0.3678	0.1698	-0.6535	0.0003	0.1299	-0.0256	-0.0655	0.2545	0.0909	0.782
24		0.6946	-0.496	0.2679	0.3817	0.0048	-0.0707	-0.2171	-0.4238	-0.0638	-0.5141	0.5137	0.802	0.2589	-0.1497	0.0371	0.338	-0.1924	-0.2163	0.1948	-0.0787	0.4396
25		0.0964	0.0116	-0.061	0.1953	-0.1572	0.055	-0.2586	-0.1066	-0.2997	-0.5379	0.1091	-0.4661	0.1358	0.0203	0	0.3858	-0.087	0.0862	0.1658	-0.0574	0.2652
26		0.1253	0.4583	-0.5761	0.3268	-0.5229	0.2607	-0.0307	-0.0341	-0.1564	0.1111	0.0266	0.037	-0.0224	0.0708	0	0.1834	0.011	0.0315	0.1727	0.0078	0.3671
27		0.2775	0.4157	-0.1531	0.2722	-0.5306	0.3395	-0.1489	0.0275	-0.4815	0.1504	0.0152	0.632	0.1737	-0.1087	-0	0.2018	-0.0437	0.0442	0.1401	-0.0308	0.4491
28		0.0078	-0.0167	0.0106	0.0189	0.0015	0.0061	0.2623	-0.3045	0.0066	0.5874	0.1273	0.219	-0.1219	0.1431	0.0008	0.6075	-0.0818	0.2579	0.3298	-0.0328	0.3963
29		0.0386	0.0076	0.0526	0.0247	-0.0888	0.1069	-0.1216	-0.1434	-0.2768	0.3242	-0.034	-0.1425	-0.0413	0.0665	0.0001	0.3237	-0.0069	0.0809	0.2487	0.0631	0.3503
30		0.214	0.0358	0.1467	-0.0245	0.3082	0.3529	0.0184	-0.181	0.0196	0.076	-0.1336	0.1012	-0.051	-0.2041	0.0002	0.1217	0.0052	-0.0162	0.1394	-0.0349	0.1263
31		0.0425	0.0344	-0.0021	0.0215	-0.0006	-0.0073	-0.2234	0.4494	-0.0428	0.0704	0.185	-0.0306	-0.3912	-0.2106	-0.0087	0.1369	0.014	-0.0504	0.1859	0.0444	0.0474
32		0.1001	-0.0759	-0.0493	0.009	-0.0544	0.0426	-0.0889	-0.1154	0.0002	-0.0657	-0.0328	0.0332	-0.068	0.026	-0	0.1115	-0.017	-0.0206	0.1788	0.0157	0.0997
33		0.0256	0.1647	0.0008	0.2954	0.0734	0.1976	-0.0338	0.0094	0.1486	-0.2021	0.0386	0.1631	-0.0931	0.0221	0	0.1659	-0.019	-0.0388	0.1306	0.0016	0.1538
34		0.0777	0.0207	0.065	0.0846	0.1318	0.1279	-0.0194	0.0644	0.0734	0.2673	0.0958	-0.1777	0.046	0.2555	0.0014	0.1435	0.0588	-0.0359	0.1338	-0.0182	0.1386
35		0.0454	-0.0605	0.123	0.1018	0.0226	0.0381	-0.193	-0.0136	0.0312	-0.0094	0.0916	-0.15	0.302	-0.0496	-0	0.1707	0.0352	0.0519	0.1292	-0.0488	0.3311
36		0.1601	0.0059	-0.2929	0.484	0.5017	0.145	-0.1826	0.0287	-0.0226	-0.0353	0.1158	-0.0351	0.0419	0.0034	-0	0.1951	-0.0062	0.0022	0.1068	-0.0204	0.1519
37		0.0095	0.0143	0.0242	0.0025	-0.0351	-0.0363	0.1891	0.041	1.0254	0.4119	0.3779	-0.5392	0.2861	0.5853	-0.0007	0.3902	-0.0002	-0.058	0.4465	-0.082	0.7011
38		0.1787	-0.1058	-0.4086	0.4141	0.0957	0.324	-0.2063	-0.0675	-0.0452	-0.0701	0.2181	-0.1769	-0.0509	-0.0217	0.0181	0.1115	-0.0096	0.0225	0.0918	-0.053	0.1824
39		0.4101	-0.0406	-0.3926	0.0191	0.0783	0.297	-0.0451	-0.2239	-0.1365	-0.0387	0.0044	-0.1297	0.0924	0.2596	-0	0.139	0.0187	-0.0158	0.1758	0.0064	0.1975
40		0.2551	-0.127	-0.014	0.1707	0.1506	0.0758	-0.015	-0.0471	-0.2145	-0.1622	0.0103	-0.039	0.1571	-0.1111	0.0001	0.1483	-0.0338	-0.0109	0.2205	0.0017	0.2237
41		-0.0386	-0.0981	0.0303	-0.0013	-0.0027	0.1161	0.1045	0.0492	-0.1256	-0.0887	-0.01	-0.0706	-0.1153	0.097	-0	0.1269	-0.0012	0.0262	0.1492	-0.0202	0.1787
42		0.3004	0.1114	0.1678	0.1344	-0.0456	0.5538	0.2925	-0.0202	0.0622	0.2676	0.1227	0.3971	-0.2437	-0.0555	0.0542	0.2335	0.0014	0.0848	0.1685	0.0108	0.1954
43		0.2146	0.0239	-0.0756	0.0064	-0.0175	0.3691	0.053	-0.0523	0.4615	0.7659	0.1561	1.0845	-0.743	0.0585	-0.0324	0.3627	0.1116	0.1795	0.1371	-0.007	0.5908
44		0.0529	0.1102	-0.0585	0.72	-0.3274	-0.1662	0.0083	0.0462	-0.0497	0.1361	0.0774	0.1155	-0.1402	0.0529	-0	0.19	-0.0244	0.0094	0.152	-0.0226	0.1807
45		-0.0042	-0.0162	-0.0092	-0.0002	0.0214	0.0346	-0.0548	-0.4393	0.266	-0.1053	-0.1906	0.027	-0.0583	-0.2096	-0.0003	0.5833	0.016	0.2847	0.3161	-0.0207	0.478
46		0.2577	-0.0963	0.3743	0.537	0.1573	0.5013	-0.2981	-0.1269	0.1504	-0.0893	0.2208	0.7438	0.1532	0.1007	-0.0002	0.1338	0.0329	0.0282	0.1219	0.0532	0.4353

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