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- PDB-3e2b: Crystal structure of Dynein Light chain LC8 in complex with a pep... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3e2b | |||||||||
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Title | Crystal structure of Dynein Light chain LC8 in complex with a peptide derived from Swallow | |||||||||
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![]() | TRANSPORT PROTEIN / protein-peptide complex / Cytoplasm / Dynein / Microtubule / Motor protein | |||||||||
Function / homology | ![]() pole plasm mRNA localization / bicoid mRNA localization / spermatid nucleus elongation / chaeta morphogenesis / regulation of pole plasm oskar mRNA localization / positive regulation of neuron remodeling / Macroautophagy / Aggrephagy / wing disc development / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand ...pole plasm mRNA localization / bicoid mRNA localization / spermatid nucleus elongation / chaeta morphogenesis / regulation of pole plasm oskar mRNA localization / positive regulation of neuron remodeling / Macroautophagy / Aggrephagy / wing disc development / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / microtubule anchoring at centrosome / chaeta development / sperm individualization / imaginal disc-derived wing morphogenesis / anterior/posterior axis specification, embryo / Neutrophil degranulation / dynein complex / dynein light intermediate chain binding / cytoplasmic dynein complex / dynein intermediate chain binding / dynein complex binding / oogenesis / establishment of mitotic spindle orientation / actin filament bundle assembly / centriole / actin filament organization / disordered domain specific binding / spermatogenesis / microtubule / cell cycle / cell division / mRNA binding / protein homodimerization activity / protein-containing complex / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Benison, G. / Barbar, E. / Karplus, P.A. | |||||||||
![]() | ![]() Title: The interplay of ligand binding and quaternary structure in the diverse interactions of dynein light chain LC8. Authors: Benison, G. / Karplus, P.A. / Barbar, E. #1: ![]() Title: Structure and dynamics of LC8 complexes with KXTQT-motif peptides: swallow and dynein intermediate chain compete for a common site. Authors: Benison, G. / Karplus, P.A. / Barbar, E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 56.3 KB | Display | ![]() |
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PDB format | ![]() | 41 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 431.9 KB | Display | ![]() |
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Full document | ![]() | 432.7 KB | Display | |
Data in XML | ![]() | 7 KB | Display | |
Data in CIF | ![]() | 8.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3briC ![]() 2p1k S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10388.849 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1783.980 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence of the peptide can be naturally found in Drosophila melanogaster (Fruit fly). References: UniProt: P40688 |
#3: Chemical | ChemComp-ACT / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.77 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 0.2M sodium potassium tartrate, 0.1M sodium citrate, 2.0M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: AREA DETECTOR / Date: Feb 27, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40.7 Å / Num. all: 10184 / Num. obs: 10184 / % possible obs: 100 % / Redundancy: 13.7 % / Biso Wilson estimate: 28.4 Å2 / Rsym value: 0.07 / Net I/σ(I): 23.5 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 14.3 % / Mean I/σ(I) obs: 5.1 / Num. unique all: 1436 / Rsym value: 0.63 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 2p1k ![]() 2p1k Resolution: 2→35.81 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.925 / SU B: 11.277 / SU ML: 0.154 / Cross valid method: THROUGHOUT / ESU R: 0.2 / ESU R Free: 0.183 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.422 Å2
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Refinement step | Cycle: LAST / Resolution: 2→35.81 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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