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- PDB-3dul: Crystal Structure Analysis of the O-methyltransferase from Bacill... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3dul | ||||||
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Title | Crystal Structure Analysis of the O-methyltransferase from Bacillus cereus | ||||||
![]() | O-methyltransferase, putative | ||||||
![]() | TRANSFERASE / alternating of alpha and beta / Methyltransferase | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cho, J.-H. / Rhee, S. | ||||||
![]() | ![]() Title: Structural and functional insights into O-methyltransferase from Bacillus cereus Authors: Cho, J.-H. / Park, Y. / Ahn, J.-H. / Lim, Y. / Rhee, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 88.8 KB | Display | ![]() |
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PDB format | ![]() | 73.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.6 KB | Display | ![]() |
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Full document | ![]() | 444.6 KB | Display | |
Data in XML | ![]() | 20 KB | Display | |
Data in CIF | ![]() | 28.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24771.361 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q739U3, Transferases; Transferring one-carbon groups; Methyltransferases #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.43 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 0.1M citric acid, 1.0M LiCl, 2% PEG 6000, 3% dioxane as an additive, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() | ||||||||||||
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 8, 2005 | ||||||||||||
Radiation | Monochromator: Mirror / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.8→50 Å / Num. all: 45976 / Num. obs: 40986 / % possible obs: 99.9 % / Observed criterion σ(F): 2 / Redundancy: 7.2 % / Rmerge(I) obs: 0.072 / Net I/σ(I): 6 | ||||||||||||
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.359 / Mean I/σ(I) obs: 4.3 / Num. unique all: 4505 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Bsol: 47.367 Å2 | ||||||||||||||||||||
Displacement parameters | Biso max: 50.98 Å2 / Biso mean: 26.86 Å2 / Biso min: 9.91 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→19.95 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.86 Å | ||||||||||||||||||||
Xplor file |
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