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- PDB-3d9i: Snapshots of the RNA processing factor SCAF8 bound to different p... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3d9i | ||||||
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Title | Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II | ||||||
![]() | RNA-binding protein 16 | ||||||
![]() | TRANSCRIPTION / SCAF8 / RNA POLYMERASE II CTD INTERACTING DOMAIN / ARM REPEATS / PHOSPHO-CTD / Phosphoprotein / RNA-binding | ||||||
Function / homology | ![]() negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled / RNA polymerase core enzyme binding / RNA polymerase II C-terminal domain phosphoserine binding / mRNA cleavage factor complex / positive regulation of DNA-templated transcription, elongation / : / termination of RNA polymerase II transcription / RNA polymerase II complex binding / nuclear matrix / mRNA binding ...negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled / RNA polymerase core enzyme binding / RNA polymerase II C-terminal domain phosphoserine binding / mRNA cleavage factor complex / positive regulation of DNA-templated transcription, elongation / : / termination of RNA polymerase II transcription / RNA polymerase II complex binding / nuclear matrix / mRNA binding / RNA binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Becker, R. / Loll, B. / Meinhart, A. | ||||||
![]() | ![]() Title: Snapshots of the RNA Processing Factor SCAF8 Bound to Different Phosphorylated Forms of the Carboxyl-terminal Domain of RNA Polymerase II. Authors: Becker, R. / Loll, B. / Meinhart, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 76.1 KB | Display | ![]() |
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PDB format | ![]() | 58.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463.9 KB | Display | ![]() |
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Full document | ![]() | 466.4 KB | Display | |
Data in XML | ![]() | 15.1 KB | Display | |
Data in CIF | ![]() | 21.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3d9jC ![]() 3d9kC ![]() 3d9lC ![]() 3d9mC ![]() 3d9nC ![]() 3d9oC ![]() 3d9pC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16925.764 Da / Num. of mol.: 2 Fragment: CTD INTERACTING DOMAIN OF SCAF8, UNP residues 1-136 Mutation: N15M, Y17M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-NH4 / #4: Chemical | ChemComp-GOL / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 52.97 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 0.1 M citric acid, 2.8 M (NH4)2SO4, 1% (v/v) glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 23, 2006 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 27084 / % possible obs: 95.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 8.2 % / Biso Wilson estimate: 29.1 Å2 / Rsym value: 0.04 / Net I/σ(I): 40 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 5.2 % / Mean I/σ(I) obs: 14.2 / Num. unique all: 5392 / Rsym value: 0.11 / % possible all: 70.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.338 Å2
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Refinement step | Cycle: LAST / Resolution: 1.907→19.62 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.907→1.956 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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