DNA polymerase V complex / homologous recombination / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / SOS response / ATP-dependent activity, acting on DNA / translesion synthesis / cell motility / single-stranded DNA binding ...DNA polymerase V complex / homologous recombination / recombinational repair / ATP-dependent DNA damage sensor activity / response to ionizing radiation / SOS response / ATP-dependent activity, acting on DNA / translesion synthesis / cell motility / single-stranded DNA binding / DNA-binding transcription factor binding / DNA recombination / damaged DNA binding / DNA damage response / ATP hydrolysis activity / ATP binding / cytoplasm / cytosol 類似検索 - 分子機能
: / : / RecA C-terminal domain / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / recA signature. / DNA recombination and repair protein RecA / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. ...: / : / RecA C-terminal domain / DNA recombination/repair protein RecA, conserved site / DNA recombination and repair protein RecA, C-terminal / recA signature. / DNA recombination and repair protein RecA / recA bacterial DNA recombination protein / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
ADENOSINE-5'-DIPHOSPHATE / TETRAFLUOROALUMINATE ION / DNA / DNA (> 10) / Protein RecA 類似検索 - 構成要素
pH: 7.5 詳細: RecA6 fusion protein was incubated with a 3-fold molar excess of ssDNA (dT)18 in the original protein buffer supplemented with 2 mM ADP, 10 mM MgCl2 and 8 mM AlF4, pH 6.0. Crystals of the ...詳細: RecA6 fusion protein was incubated with a 3-fold molar excess of ssDNA (dT)18 in the original protein buffer supplemented with 2 mM ADP, 10 mM MgCl2 and 8 mM AlF4, pH 6.0. Crystals of the RecA6-(ADP-AlF4-Mg)6-(dT)18 complex were grown from 50 mM Hepes-Na+, 1.5% (w/v) polyethylene glycol (PEG) 3350, 4% (w/v) polyvinylpyrrolidone K15 (PVP K15), 25% (v/v) 2-methyl-2,4-pentandiol (MPD), 10 mM DTT, pH 7.5.
溶液の組成
ID
名称
Crystal-ID
Sol-ID
1
ADP
1
1
2
MgCl2
1
1
3
AlF4
1
1
4
Hepes-Na+
1
1
5
PEG3350
1
1
6
PVPK15
1
1
7
MPD
1
1
8
DTT
1
1
9
Hepes-Na+
1
2
10
PEG3350
1
2
11
PVPK15
1
2
12
MPD
1
2
13
DTT
1
2
-
データ収集
放射光源
由来: シンクロトロン / サイト: APS / ビームライン: 24-ID-C
検出器
日付: 2006年10月1日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
相対比: 1
反射
解像度: 4.2→40 Å / Num. obs: 16125
-
解析
ソフトウェア
名称: REFMAC / バージョン: 5.2.0019 / 分類: 精密化
精密化
構造決定の手法: 分子置換 / 解像度: 4.2→12 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.922 / SU B: 197.085 / SU ML: 1.083 / 交差検証法: THROUGHOUT / ESU R Free: 1.328 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD
Rfactor
反射数
%反射
Selection details
Rfree
0.298
905
5.3 %
RANDOM
Rwork
0.275
-
-
-
obs
0.276
16125
80.7 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: BABINET MODEL WITH MASK