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Open data
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Basic information
Entry | Database: PDB / ID: 3blx | ||||||
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Title | Yeast Isocitrate Dehydrogenase (Apo Form) | ||||||
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![]() | OXIDOREDUCTASE / TCA cycle / oxidative metabolism / allostery / dehydrogenase / decarboxylase / Allosteric enzyme / Magnesium / Manganese / Metal-binding / Mitochondrion / NAD / RNA-binding / Transit peptide / Tricarboxylic acid cycle / Phosphoprotein | ||||||
Function / homology | ![]() Citric acid cycle (TCA cycle) / : / isocitrate dehydrogenase (NAD+) / isocitrate dehydrogenase (NAD+) activity / glutamate biosynthetic process / isocitrate metabolic process / mitochondrial nucleoid / tricarboxylic acid cycle / mitochondrial intermembrane space / NAD binding ...Citric acid cycle (TCA cycle) / : / isocitrate dehydrogenase (NAD+) / isocitrate dehydrogenase (NAD+) activity / glutamate biosynthetic process / isocitrate metabolic process / mitochondrial nucleoid / tricarboxylic acid cycle / mitochondrial intermembrane space / NAD binding / mitochondrial matrix / magnesium ion binding / mitochondrion / RNA binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Taylor, A.B. / Hu, G. / Hart, P.J. / McAlister-Henn, L. | ||||||
![]() | ![]() Title: Allosteric Motions in Structures of Yeast NAD+-specific Isocitrate Dehydrogenase. Authors: Taylor, A.B. / Hu, G. / Hart, P.J. / McAlister-Henn, L. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 988.4 KB | Display | ![]() |
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PDB format | ![]() | 827.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 586.4 KB | Display | ![]() |
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Full document | ![]() | 779.5 KB | Display | |
Data in XML | ![]() | 192.9 KB | Display | |
Data in CIF | ![]() | 257.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3blvSC ![]() 3blwC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38076.352 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: IDH1 / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: ![]() ![]() References: UniProt: P28834, isocitrate dehydrogenase (NAD+) #2: Protein | Mass: 37839.027 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: IDH2 / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: ![]() ![]() References: UniProt: P28241, isocitrate dehydrogenase (NAD+) |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.21 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 16% polyethylene glycol 3350, 0.13 M potassium sulfate, 0.2 M trimethylamine-N-oxide, 15% propanediol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 11, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. obs: 185029 / % possible obs: 96.6 % / Redundancy: 3.6 % / Biso Wilson estimate: 71.3 Å2 / Rsym value: 0.078 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 2 / Num. unique all: 18017 / Rsym value: 0.554 / % possible all: 94.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3BLV Resolution: 2.7→35.739 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT
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Displacement parameters | Biso mean: 58.2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.7→35.739 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.7307 Å
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