- PDB-3bcq: Crystal structure of oxy-hemoglobin from Brycon cephalus -
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IDまたはキーワード:
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基本情報
登録情報
データベース: PDB / ID: 3bcq
タイトル
Crystal structure of oxy-hemoglobin from Brycon cephalus
要素
Alpha-chain hemoglobin
Beta-chain hemoglobin
キーワード
TRANSPORT PROTEIN/OXYGEN BINDING / hemoglobin / fish / brycon cephalus / Heme / Iron / Metal-binding / Oxygen transport / Transport / TRANSPORT PROTEIN-OXYGEN BINDING COMPLEX
機能・相同性
機能・相同性情報
hemoglobin complex / oxygen carrier activity / oxygen binding / heme binding / metal ion binding 類似検索 - 分子機能
ACCORDING TO THE AUTHOR, THESE DIFFERENCES IN ALPHA CHAIN, BETWEEN THE DATABASE AND SEQRES, CAME ...ACCORDING TO THE AUTHOR, THESE DIFFERENCES IN ALPHA CHAIN, BETWEEN THE DATABASE AND SEQRES, CAME FROM SOME MISTAKES IN THE PREVIOUS DNA SEQUENCING. THE SEQUENCE PRESENTED IN THE SEQRES WAS CONFIRMED BY A NEW DNA SEQUENCING (N=3), HOWEVER THESE DATA WAS NOT REFRESHED YET. THERE IS NO UNP REFERENCE SEQUENCE DATABASE FOR BETA-CHAIN HEMOGLOBIN AT THE TIME OF PROCESSING.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 2
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試料調製
結晶
マシュー密度: 2.36 Å3/Da / 溶媒含有率: 47.88 %
結晶化
温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5 詳細: pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
解像度: 2.4→19.89 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.895 / SU B: 8.924 / SU ML: 0.213 / 交差検証法: THROUGHOUT / ESU R: 1.948 / ESU R Free: 0.356 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.27944
1042
5.1 %
RANDOM
Rwork
0.1662
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obs
0.17175
19316
86.93 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK