[English] 日本語
![](img/lk-miru.gif)
- PDB-3azs: Diverse Substrates Recognition Mechanism Revealed by Thermotoga m... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3azs | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Diverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with Mannotriose | |||||||||
![]() | Endoglucanase | |||||||||
![]() | HYDROLASE / cellulose / cellulase / biofuel / Tim Barrel | |||||||||
Function / homology | ![]() glucan catabolic process / beta-glucosidase activity / cell surface / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Wu, T.H. / Huang, C.H. / Ko, T.P. / Lai, H.L. / Ma, Y. / Chen, C.C. / Cheng, Y.S. / Liu, J.R. / Guo, R.T. | |||||||||
![]() | ![]() Title: Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose Authors: Wu, T.H. / Huang, C.H. / Ko, T.P. / Lai, H.L. / Ma, Y. / Chen, C.C. / Cheng, Y.S. / Liu, J.R. / Guo, R.T. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 155.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 121.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 31.2 KB | Display | |
Data in CIF | ![]() | 48 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3amcSC ![]() 3amdC ![]() 3amgC ![]() 3aofC ![]() 3azrC ![]() 3aztC S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 37380.488 Da / Num. of mol.: 2 / Mutation: E253A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.83 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.1M tris pH8.5 , 0.4M NaCl, 28% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 20, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.69→25 Å / Num. obs: 67472 / % possible obs: 99.4 % / Observed criterion σ(I): 2 / Redundancy: 4.1 % / Rsym value: 0.043 / Net I/σ(I): 30.1 |
Reflection shell | Resolution: 1.69→1.75 Å / Redundancy: 4 % / Mean I/σ(I) obs: 10.6 / Num. unique all: 6608 / Rsym value: 0.124 / % possible all: 97.4 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3AMC Resolution: 1.69→25 Å / σ(F): 2 / Stereochemistry target values: ML
| ||||||||||||||||||||
Solvent computation | Bsol: 44.6583 Å2 | ||||||||||||||||||||
Displacement parameters | Biso mean: 15.2534 Å2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.69→25 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
Xplor file |
|