[English] 日本語
![](img/lk-miru.gif)
- PDB-3a71: High resolution structure of Penicillium chrysogenum alpha-L-arab... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3a71 | ||||||
---|---|---|---|---|---|---|---|
Title | High resolution structure of Penicillium chrysogenum alpha-L-arabinanase | ||||||
![]() | Exo-arabinanase | ||||||
![]() | HYDROLASE / ARABINASE / GLYCOSYL HYDROLASE | ||||||
Function / homology | Neuraminidase - #10 / 6 Propeller / Neuraminidase / Mainly Beta / ACETATE ION / Exo-arabinanase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sogabe, Y. | ||||||
![]() | ![]() Title: High-resolution structure of exo-arabinanase from Penicillium chrysogenum Authors: Sogabe, Y. / Kitatani, T. / Yamaguchi, A. / Kinoshita, T. / Adachi, H. / Takano, K. / Inoue, T. / Mori, Y. / Matsumura, H. / Sakamoto, T. / Tada, T. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 170.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 133.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 441.7 KB | Display | |
Data in XML | ![]() | 19.7 KB | Display | |
Data in CIF | ![]() | 31.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 39359.625 Da / Num. of mol.: 1 / Fragment: UNP Residues 24-378 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q7ZA77, non-reducing end alpha-L-arabinofuranosidase | ||
---|---|---|---|
#2: Chemical | ChemComp-ACT / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.81 % |
---|---|
Crystal grow | Temperature: 277 K / pH: 4.5 Details: 28% MPD, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 13, 2006 | ||||||||||||
Radiation | Monochromator: ROTATED-INCLINED DOUBLE-CRYSTAL MO / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
| ||||||||||||
Reflection | Resolution: 1.14→10 Å / Num. obs: 149407 / % possible obs: 99.7 % / Redundancy: 7.2 % / Biso Wilson estimate: 10.7 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 32 | ||||||||||||
Reflection shell | Resolution: 1.14→1.18 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.342 / Mean I/σ(I) obs: 5.4 / % possible all: 99 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 8.17 Å2
| |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 2652 / Occupancy sum non hydrogen: 3336
| |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.14→10 Å
| |||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.14→1.17 Å / Total num. of bins used: 20
|