LUMINESCENT PROTEIN / GFP-LIKE PROTEIN / Structural Genomics / RIKEN Structural Genomics/Proteomics Initiative / RSGI / NPPSFA / National Project on Protein Structural and Functional Analyses
Function / homology
Green Fluorescent Protein / Green fluorescent protein / Beta Barrel / Mainly Beta
Mass: 18.015 Da / Num. of mol.: 319 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THERE IS NO UNP REFERENCE SEQUENCE DATABASE FOR THIS PROTEIN AT THE TIME OF PROCESSING. RESIDUES -2- ...THERE IS NO UNP REFERENCE SEQUENCE DATABASE FOR THIS PROTEIN AT THE TIME OF PROCESSING. RESIDUES -2-0 AND 221 ARE EXPRESSION TAGS. THE DIFFERENCE BETWEEN THE SEQUENCE DATABASE THAT WILL BE DEPOSITED AT A LATER TIME AND THE COORDINATES IS THAT GYS 64 REPRESENTS THE CHROMOPHORE (SER-TYR-GLY). RESIDUES SER 63, TYR 64 AND GLY 65 ARE LABELLED AS RESIDUE 64 IN THE COORDINATES.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.23 Å3/Da / Density % sol: 44.81 %
Crystal grow
Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 35-40% PEG 8000, 0.2M (NH4)2SO4, 0.1M SODIUM CACODYLATE, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 291K
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