+Open data
-Basic information
Entry | Database: PDB / ID: 2znz | ||||||
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Title | Crystal structure of FFRP | ||||||
Components | Uncharacterized HTH-type transcriptional regulator PH1519 | ||||||
Keywords | DNA BINDING PROTEIN / transcription / DNA-binding / Transcription regulation | ||||||
Function / homology | Function and homology information response to amino acid / sequence-specific DNA binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Pyrococcus horikoshii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.39 Å | ||||||
Authors | Yamada, M. / Suzuki, M. | ||||||
Citation | Journal: Proteins / Year: 2009 Title: Interactions between the archaeal transcription repressor FL11 and its coregulators lysine and arginine. Authors: Yamada, M. / Ishijima, S.A. / Suzuki, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2znz.cif.gz | 242 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2znz.ent.gz | 196.2 KB | Display | PDB format |
PDBx/mmJSON format | 2znz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2znz_validation.pdf.gz | 510.8 KB | Display | wwPDB validaton report |
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Full document | 2znz_full_validation.pdf.gz | 525.4 KB | Display | |
Data in XML | 2znz_validation.xml.gz | 48 KB | Display | |
Data in CIF | 2znz_validation.cif.gz | 65.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zn/2znz ftp://data.pdbj.org/pub/pdb/validation_reports/zn/2znz | HTTPS FTP |
-Related structure data
Related structure data | 2znyC 1ri7S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 19151.221 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus horikoshii (archaea) / Strain: OT3 / Gene: fl11, PH1519 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O59188 #2: Chemical | ChemComp-LYS / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.17 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 20% PEG 3350, 0.3M CaCl2, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Oct 17, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.39→50 Å / Num. all: 71589 / Num. obs: 71303 / % possible obs: 99.6 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Rmerge(I) obs: 0.087 / Net I/σ(I): 17.47 |
Reflection shell | Resolution: 2.39→2.48 Å / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 4.83 / % possible all: 99.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1RI7 Resolution: 2.39→46.3 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.39→46.3 Å
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