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- PDB-2znx: 5-Fluorotryptophan Incorporated ScFv10 Complexed to Hen Egg Lysozyme -
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Open data
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Basic information
Entry | Database: PDB / ID: 2znx | ||||||
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Title | 5-Fluorotryptophan Incorporated ScFv10 Complexed to Hen Egg Lysozyme | ||||||
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![]() | IMMUNE SYSTEM/HYDROLASE / fluorotryptohpan / 5-fluorotryptophan / 19F / Single Chain Fv / Lysozyme / Allergen / Antimicrobial / Bacteriolytic enzyme / Glycosidase / Hydrolase / IMMUNE SYSTEM-HYDROLASE COMPLEX | ||||||
Function / homology | ![]() immunoglobulin complex / Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / RNA-directed DNA polymerase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium ...immunoglobulin complex / Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / RNA-directed DNA polymerase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / adaptive immune response / killing of cells of another organism / defense response to Gram-positive bacterium / defense response to bacterium / immune response / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() synthetic construct (others) ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | DeSantis, M.E. / Acchione, M. / Li, M. / Walter, R.L. / Wlodawer, A. / Smith-Gill, S. | ||||||
![]() | ![]() Title: Specific fluorine labeling of the HyHEL10 antibody affects antigen binding and dynamics Authors: Acchione, M. / Lee, Y.C. / DeSantis, M.E. / Lipschultz, C.A. / Wlodawer, A. / Li, M. / Shanmuganathan, A. / Walter, R.L. / Smith-Gill, S. / Barchi, J.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 155.5 KB | Display | ![]() |
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PDB format | ![]() | 122.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2znwC ![]() 2dqjS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Refine code: 4
NCS ensembles :
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Components
#1: Antibody | Mass: 26270.408 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: This molecule contains antibody light chain(residues 1-107), linker(residues 108-122) and antibody heavy chain(residues 123-236) Source: (gene. exp.) ![]() ![]() Plasmid: pET30 / Production host: ![]() ![]() #2: Protein | Mass: 14331.160 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.78 Å3/Da / Density % sol: 55.79 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 30% PEG400, 0.2M Na Citrate; 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Dec 18, 2007 / Details: mirrors |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 47378 / Num. obs: 47378 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 9 % / Rsym value: 0.12 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 8.8 % / Mean I/σ(I) obs: 4.2 / Num. unique all: 4648 / Rsym value: 0.786 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2DQJ Resolution: 2.3→50 Å / Cor.coef. Fo:Fc: 0.94 / Cor.coef. Fo:Fc free: 0.908 / SU B: 5.36 / SU ML: 0.135 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.272 / ESU R Free: 0.209 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.604 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→50 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 2.3→2.36 Å / Total num. of bins used: 20
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