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- PDB-2zca: Crystal Structure of TTHB189, a CRISPR-associated protein, Cse2 f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2zca | ||||||
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Title | Crystal Structure of TTHB189, a CRISPR-associated protein, Cse2 family from Thermus thermophilus HB8 | ||||||
![]() | Putative uncharacterized protein TTHB189 | ||||||
![]() | Structural Genomics / UNKNOWN FUNCTION / CRISPR / Cse2 / Thermus thermophilus / Plasmid / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | CRISPR-associated protein Cse2 / CRISPR-associated protein Cse2 / Cse2 superfamily / CRISPR-associated protein Cse2 (CRISPR_cse2) / Peroxidase; domain 1 / defense response to virus / Orthogonal Bundle / Mainly Alpha / CRISPR-associated protein Cse2![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Agari, Y. / Shinkai, A. / Yokoyama, S. / Kuramitsu, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: X-ray crystal structure of a CRISPR-associated protein, Cse2, from Thermus thermophilus HB8 Authors: Agari, Y. / Yokoyama, S. / Kuramitsu, S. / Shinkai, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.2 KB | Display | ![]() |
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PDB format | ![]() | 60.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422.1 KB | Display | ![]() |
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Full document | ![]() | 424 KB | Display | |
Data in XML | ![]() | 15.3 KB | Display | |
Data in CIF | ![]() | 22.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19529.867 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.9 Details: 30% PEG 600, 5% PEG 1000, 0.1M Sodium Cacodylate, 10% Glycerol, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RIGAKU JUPITER 210 / Detector: CCD / Date: Jul 18, 2007 Details: two dimensional focusing mirror which is coated in rhodium. |
Radiation | Monochromator: A fixed exit Si double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97897 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. all: 32767 / Num. obs: 32767 / % possible obs: 99.9 % / Redundancy: 5.5 % / Biso Wilson estimate: 14.8 Å2 / Rmerge(I) obs: 0.071 / Net I/σ(I): 27.8 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 5.4 % / Rmerge(I) obs: 0.278 / Mean I/σ(I) obs: 3.92 / Num. unique all: 3255 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.216 Å2 / ksol: 0.370733 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→39.17 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.011 / Total num. of bins used: 6
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Xplor file |
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