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- PDB-2zae: Crystal structure of protein Ph1601p in complex with protein Ph17... -

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Basic information

Entry
Database: PDB / ID: 2zae
TitleCrystal structure of protein Ph1601p in complex with protein Ph1771p of archaeal ribonuclease P from Pyrococcus horikoshii OT3
Components(Ribonuclease P protein component ...) x 2
KeywordsHYDROLASE / Ribonuclease P Protein subunits / hetero dimer / tRNA processing
Function / homology
Function and homology information


ribonuclease P complex / ribonuclease P / ribonuclease P activity / tRNA 5'-leader removal / RNA binding / zinc ion binding / cytoplasm
Similarity search - Function
N-terminal domain of TfIIb - #20 / Ribonuclease P/MRP, subunit p29 / Ribonuclease P subunit RNP4 / Ribonuclease P protein subunit RNP1 / Ribonuclease P subunit, Rpr2/Snm1/Rpp21 / RNAse P Rpr2/Rpp21/SNM1 subunit domain / Ribonuclease P protein subunit Rpp29/RNP1 / Ribonuclease P/MRP subunit Rpp29 superfamily / Ribonuclease P/MRP, subunit p29 / A domain found in a protein subunit of human RNase MRP and RNase P ribonucleoprotein complexes and archaeal proteins. ...N-terminal domain of TfIIb - #20 / Ribonuclease P/MRP, subunit p29 / Ribonuclease P subunit RNP4 / Ribonuclease P protein subunit RNP1 / Ribonuclease P subunit, Rpr2/Snm1/Rpp21 / RNAse P Rpr2/Rpp21/SNM1 subunit domain / Ribonuclease P protein subunit Rpp29/RNP1 / Ribonuclease P/MRP subunit Rpp29 superfamily / Ribonuclease P/MRP, subunit p29 / A domain found in a protein subunit of human RNase MRP and RNase P ribonucleoprotein complexes and archaeal proteins. / Rof/RNase P-like / N-terminal domain of TfIIb / Immunoglobulin FC, subunit C / Other non-globular / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Special / SH3 type barrels. / Roll / Up-down Bundle / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
NITRATE ION / Ribonuclease P protein component 4 / Ribonuclease P protein component 1
Similarity search - Component
Biological speciesPyrococcus horikoshii (archaea)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.21 Å
AuthorsHonda, T. / Kakuta, Y. / Kimura, M.
CitationJournal: J.Mol.Biol. / Year: 2008
Title: Structure of an archaeal homolog of the human protein complex Rpp21-Rpp29 that is a key core component for the assembly of active ribonuclease P.
Authors: Honda, T. / Kakuta, Y. / Kimura, K. / Saho, J. / Kimura, M.
History
DepositionOct 4, 2007Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Oct 14, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.2Nov 1, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ribonuclease P protein component 1
B: Ribonuclease P protein component 4
C: Ribonuclease P protein component 1
D: Ribonuclease P protein component 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,45219
Polymers59,3954
Non-polymers1,05715
Water3,225179
1
A: Ribonuclease P protein component 1
B: Ribonuclease P protein component 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,28710
Polymers29,6972
Non-polymers5908
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Ribonuclease P protein component 1
D: Ribonuclease P protein component 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,1659
Polymers29,6972
Non-polymers4687
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)74.829, 127.070, 51.857
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11B-150-

HOH

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Components

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Ribonuclease P protein component ... , 2 types, 4 molecules ACBD

#1: Protein Ribonuclease P protein component 1 / RNase P component 1


Mass: 15070.599 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pyrococcus horikoshii (archaea) / Strain: OT3 / Gene: rnp1 / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)CodonPlus RIL / References: UniProt: O59425, ribonuclease P
#2: Protein Ribonuclease P protein component 4 / RNase P component 4


Mass: 14626.764 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Pyrococcus horikoshii (archaea) / Strain: OT3 / Gene: rnp4 / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)CodonPlus RIL / References: UniProt: O59248, ribonuclease P

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Non-polymers , 4 types, 194 molecules

#3: Chemical
ChemComp-NO3 / NITRATE ION


Mass: 62.005 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: NO3
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#5: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 179 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.08 Å3/Da / Density % sol: 40.74 %
Crystal growTemperature: 293.4 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 20% PEG 3350, 0.2M KNO3, 50mM NaCl,50mM Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.4K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL38B1 / Wavelength: 1 Å
DetectorType: RIGAKU JUPITER 210 / Detector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.2→50 Å / Num. all: 25221 / Num. obs: 25221 / % possible obs: 98.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6 % / Rmerge(I) obs: 0.064 / Rsym value: 0.064 / Net I/σ(I): 33.8
Reflection shellResolution: 2.2→2.28 Å / Redundancy: 5.4 % / Rmerge(I) obs: 0.369 / Mean I/σ(I) obs: 3.15 / Num. unique all: 1552 / Rsym value: 0.369 / % possible all: 89.6

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Processing

Software
NameVersionClassification
REFMAC5.2.0019refinement
BSSdata collection
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1X0T, 1V76
Resolution: 2.21→42.64 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.93 / SU B: 11.868 / SU ML: 0.159 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.278 / ESU R Free: 0.219 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.25005 1273 5 %RANDOM
Rwork0.19938 ---
obs0.20191 23939 98.52 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 36.98 Å2
Baniso -1Baniso -2Baniso -3
1--0.17 Å20 Å20 Å2
2---0.08 Å20 Å2
3---0.25 Å2
Refinement stepCycle: LAST / Resolution: 2.21→42.64 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3365 0 62 179 3606
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.0223495
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg1.3971.9774675
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.2855406
X-RAY DIFFRACTIONr_dihedral_angle_2_deg28.60720.065153
X-RAY DIFFRACTIONr_dihedral_angle_3_deg18.92815686
X-RAY DIFFRACTIONr_dihedral_angle_4_deg19.1171553
X-RAY DIFFRACTIONr_chiral_restr0.1110.2503
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.022557
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined0.2170.21435
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined0.310.22303
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1510.2179
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.2290.279
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.2140.213
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.6511.52113
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it1.10423292
X-RAY DIFFRACTIONr_scbond_it1.60631567
X-RAY DIFFRACTIONr_scangle_it2.464.51381
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.207→2.264 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.307 73 -
Rwork0.232 1552 -
obs--87.84 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
110.0263-2.35772.68815.8309-2.66183.5777-0.226-0.26340.18310.4540.1246-0.1865-0.5492-0.13210.1014-0.00250.0285-0.0330.08890.01740.104211.564-14.6559-6.7273
24.0035-1.8971-1.02275.690.06033.8416-0.17490.0799-0.121-0.24790.0273-0.17880.39740.23940.14750.07320.08750.04050.06740.03270.139526.4866-25.1646-12.9144
33.90182.1607-4.00331.2374-1.577714.11860.2729-0.74570.2360.90490.06730.0257-1.57490.2935-0.34030.20170.0657-0.0230.05580.0230.068324.5567-23.0356.4743
48.56081.63270.05434.6256-1.27555.11340.0938-0.26690.04080.1935-0.1508-0.44-0.34230.24050.05710.0380.0425-0.00970.06310.01170.135124.4662-16.2732-3.8103
54.6467-0.96041.04462.6344-2.19412.0215-0.0544-0.32110.02630.25170.0902-0.1118-0.2648-0.1866-0.03580.02420.0317-0.05960.1239-0.01130.109122.6566-17.07432.3231
64.54252.41360.035610.0203-2.57325.2661-0.24660.14950.1275-0.56570.0882-0.52130.1710.61230.15840.00230.0604-0.01760.06830.02280.113130.7986-23.7135-7.1629
711.5908-3.69311.90185.6876-1.69785.40430.07560.9701-0.2196-0.4859-0.5331-0.5860.36361.20380.45750.0610.05670.03660.16640.09940.072330.0963-21.0431-21.8751
87.4669-2.2851-1.53933.6688-2.24315.6793-0.0185-0.10970.2349-0.0906-0.0091-0.0966-0.20630.02220.02760.09040.0127-0.02290.05320.02740.121218.4642-10.7524-15.8934
916.96331.0905-0.038112.0489-2.69195.11740.25511.35910.8056-0.7107-0.1805-0.3802-0.12180.6072-0.07460.15950.11080.14140.14020.1329-0.006630.0467-13.4471-26.9725
101.05180.24612.2042.15520.80814.6590.3061-0.0287-0.06090.0438-0.1432-0.15430.52960.0481-0.1630.1265-0.0449-0.04420.0560.03920.092215.7195-7.8418-25.0865
1111.1928-4.97881.8674.36261.3222.46850.80451.59840.194-1.0178-0.4667-0.3111-0.2583-0.0008-0.33780.21780.02740.03670.12890.01480.07716.9243-2.9114-40.3034
129.7521.0972-0.01984.92383.127910.17640.02530.5405-0.2852-0.21030.04250.00470.28470.125-0.06780.0919-0.0214-0.02650.05220.02630.122813.0114-2.0252-31.6619
139.3408-1.1032-1.01812.49522.40693.38910.23980.03330.56570.2912-0.4551-0.0308-0.61790.00670.21530.05680.03030.02340.13760.01420.104274.1782-12.3751-6.0188
146.35431.90092.09394.3555-1.48073.7681-0.13440.2812-0.3026-0.13970.27340.18950.4501-0.3286-0.1390.1052-0.0950.02320.10180.00370.078256.8706-24.921-4.4423
151.54280.89242.84327.79640.131322.4312-0.2463-0.1216-0.3240.01830.05020.03190.12220.42590.19610.0414-0.0976-0.01190.07710.01070.14161.6561-19.9786-6.1639
161.0722-1.08910.48752.1562-0.84034.7909-0.0170.0724-0.0159-0.2097-0.00750.2241-0.02750.02530.02450.0199-0.0597-0.00250.08660.0440.135960.2965-12.5657-14.9795
176.39170.51661.49460.1154-0.5326.13220.09820.11250.3335-0.3041-0.05580.04140.0437-0.0467-0.04240.01920.0128-0.00640.09250.03090.140561.8014-11.0026-14.2827
184.0968-1.718-0.23425.7513-0.28474.7055-0.0894-0.00990.0526-0.13080.28380.37870.1054-0.5238-0.1945-0.0203-0.088-0.0230.14630.09130.127253.9479-19.1696-8.8371
1921.173111.70077.48028.54947.499433.89291.039-1.00260.27020.999-0.66821.1823-0.4817-3.0444-0.3708-0.1102-0.07860.15670.27530.07370.179747.5665-25.37856.321
205.33921.5209-0.14581.8488-0.1782.61810.0233-0.12070.06270.17750.01350.0794-0.0379-0.0438-0.03690.06680.0020.01630.0997-0.00180.112764.961-15.79854.1518
2127.0896-14.25743.774313.6045-0.71289.28990.0114-0.9198-0.38120.47230.15350.53780.1542-1.1022-0.16480.0452-0.10360.11480.080.01340.080655.6677-18.95412.6159
221.50250.1125-0.12564.0475-0.22092.34570.2551-0.14830.03440.1267-0.07770.03480.3163-0.0321-0.17750.0658-0.00730.01070.0930.01310.098570.158-14.156614.703
232.21731.5314-3.26762.5571-6.027114.2962-0.1192-0.56050.21510.23780.0308-0.08350.99760.98750.08830.48420.08180.1071-0.0133-0.00640.14473.0956-21.093631.1502
246.10871.8465-2.42933.6641-2.65524.9437-0.1704-0.2338-0.34790.14890.2276-0.08540.91460.0368-0.05720.23330.00640.01950.0478-0.00970.053170.4612-14.487222.842
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1AA18 - 3018 - 30
2X-RAY DIFFRACTION2AA31 - 5231 - 52
3X-RAY DIFFRACTION3AA53 - 6653 - 66
4X-RAY DIFFRACTION4AA67 - 8067 - 80
5X-RAY DIFFRACTION5AA81 - 9381 - 93
6X-RAY DIFFRACTION6AA94 - 12294 - 122
7X-RAY DIFFRACTION7BB11 - 2811 - 28
8X-RAY DIFFRACTION8BB29 - 5129 - 51
9X-RAY DIFFRACTION9BB52 - 6152 - 61
10X-RAY DIFFRACTION10BB62 - 8162 - 81
11X-RAY DIFFRACTION11BB82 - 9282 - 92
12X-RAY DIFFRACTION12BB93 - 10893 - 108
13X-RAY DIFFRACTION13CC17 - 2917 - 29
14X-RAY DIFFRACTION14CC30 - 4630 - 46
15X-RAY DIFFRACTION15CC47 - 5247 - 52
16X-RAY DIFFRACTION16CC53 - 7653 - 76
17X-RAY DIFFRACTION17CC77 - 9577 - 95
18X-RAY DIFFRACTION18CC96 - 12196 - 121
19X-RAY DIFFRACTION19DD12 - 1712 - 17
20X-RAY DIFFRACTION20DD18 - 5118 - 51
21X-RAY DIFFRACTION21DD52 - 6252 - 62
22X-RAY DIFFRACTION22DD63 - 8263 - 82
23X-RAY DIFFRACTION23DD83 - 9283 - 92
24X-RAY DIFFRACTION24DD93 - 10993 - 109

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